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目的显微镜下建立一种能很好模拟人体内生物学行为的胃癌裸小鼠原位移植和转移模型.方法采用人胃癌SGC-7901细胞株在裸鼠皮下反复传5代形成实体瘤,显微镜下将人胃癌裸鼠皮下移植瘤块种植于裸小鼠胃壁,建立胃癌裸小鼠原位移植和转移模型16个,观察建立的模型肿瘤生长状况、移植成功率和自发转移的发生率,常规HE染色,观察胃癌原位移植瘤、胃癌淋巴结转移和肝转移的病理切片,电镜下观察肿瘤超微结构.结果胃癌裸鼠皮下移植瘤原位移植的原位成瘤率达100%(16/16),淋巴结广泛转移率为87.5%(14/16),肝转移发生率为75%(12/16),腹水形成率为12.5%(2/16).结论显微镜下建立原位移植和转移模型成功率高,且具有人胃癌自然生长过程的特点和生物学活性.“,”Objective To develop a nude mouse model of human gastric cancer which can mimic its natural human biologic activities under the microscope. Methods Human gastric cancer SGC-7901 was cultured in nude mice repeatedly for 5 generations to get the Tumor subcutaneous solid tumor, which was then emulsified and orthotopicly implanted into the nude mouse stomach under the microscope to develop 16 implant-tumors. The tumor growth characteristic, tumor-take rates and metastatic rates were analyzed grossly. The gastric orthotopicaly transplantation, gastric cancer lymphatic metastasis and hepatic metastasis by HE staining, the ultrostructures, by electron microscopy were analyzed too.Results The tumor-take rate was 100%, the metastases of lymph nodes and livers were 87.5% and 75%, ascites formation is 12.5%. Conclusion To repeat the model under the microscope is easy and qualified, which can exhibiting natural growth characters and biological activities successfuly.