论文部分内容阅读
目的对一起幼儿园聚集性发热事件开展病原学调查,为疫情处置提供依据。方法采集部分患者呼吸道样品,应用real-time RT-PCR法筛查,腺病毒核酸阳性样品接种HEp-2细胞系,PCR扩增部分腺病毒Hexon高变区基因片段并测序,确定病毒型别并进行变异分析。结果从10份咽拭子标本中检测到7份腺病毒核酸阳性。经分离培养得到7株人腺病毒毒株。系统进化分析确定均为人腺病毒7型。推导的氨基酸序列分析显示,与近年来国内外流行株相比,分离株的Hexon蛋白高变区无氨基酸位点突变。结论结合病例临床表现以及流行病学调查结果,此事件证实系人腺病毒7型引起的急性呼吸道感染聚集性事件。
Objective To conduct an etiological investigation of a kindergarten gathering fever and provide a basis for the outbreak treatment. Methods The respiratory samples of some patients were collected and screened by real-time RT-PCR. The positive samples of adenovirus were inoculated into HEp-2 cell line and amplified by PCR. Perform mutation analysis. Results Seven adenovirus positive were detected in 10 throat swab specimens. After isolation and culture to obtain seven strains of human adenovirus. Phylogenetic analysis showed that all of them were human adenovirus type 7. The deduced amino acid sequence analysis showed that there was no amino acid site mutation in the hypervariable region of the Hexon protein of the isolate compared with the epidemic strains at home and abroad in recent years. Conclusion Combined with the clinical manifestations and epidemiological findings, this incident confirmed the human Adenovirus type 7 caused by acute respiratory infections aggregated events.