论文部分内容阅读
报道了使用阳离子双子表面活性剂作为毛细管电泳的缓冲添加剂用于同时分离酸性和碱性蛋白质.在酸性的缓冲条件下,只需要使用低浓度的阳离子双子表面活性剂(0.1mmol/L18-s-18)作为缓冲液的添加剂,就可以有效地抑制酸性和碱性蛋白质在毛细管壁的吸附,从而得到高效的蛋白质分离.实验表明,较小的胶束尺寸(如s=5～8)比大的胶束尺寸(如s<4或>10)能更有效地抑制酸性蛋白质的吸附.改变双子表面活性剂的中间基的长度能够对蛋白质的电泳淌度进行一定的调节,从而对分离的选择性进行一定的优化.在最优的实验条件下,蛋白质迁移时间的日内和日间标准偏差(RSD)分别小于0.8%和2.2%,回收率为79%到100.4%.另外,还考察了双子表面活性剂保护的金纳米颗粒用作毛细管电泳缓冲添加剂在蛋白质分离中的应用.实验表明,在缓冲液中加入纳米金能够缩短分析时间,并能小幅度地提高分离效率.最后,使用该方法分析了一系列复杂生物样品,包括血浆、红细胞和鸡蛋清样品,均得到了满意的结果.
Reported the use of cationic gemini surfactant as a buffer additive for capillary electrophoresis for the simultaneous separation of acidic and basic proteins using only low concentrations of cationic gemini surfactant (0.1 mmol / L 18-s-1) 18) as a buffer solution can effectively inhibit the adsorption of acidic and basic proteins on the capillary wall to obtain efficient protein separation.Experiments show that smaller micelles size (such as s = 5 ~ 8) than large (Such as s <4 or> 10) can more effectively inhibit the adsorption of acidic proteins.Changing the length of the gemini gels can adjust the electrophoretic mobility of the proteins to some extent, The optimal conditions were as follows: the intra-and inter-day standard deviation (RSD) of protein migration time were less than 0.8% and 2.2%, respectively, and the recoveries were between 79% and 100.4% under the optimal experimental conditions.In addition, The use of surfactant-protected gold nanoparticles as a capillary electrophoresis buffer additive in protein separation.Experiments have shown that the addition of gold nanoparticles to the buffer can shorten the analysis time and provide a small improvement High separation efficiency.Finally, the method was used to analyze a series of complex biological samples, including plasma, erythrocyte and egg white samples, with satisfactory results.