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目的探讨rhIL-11对中子照射后IEC-6细胞IL-11Rα和gp130表达的影响。方法培养的IEC-6细胞经4·0Gy中子照射并于照前12h与照后即刻给予100ng/ml的rhIL-11,采用流式细胞术、免疫组化、Westernblot及图像分析等技术检测IEC-6细胞的凋亡和坏死率、IL-11Rα和gp130的表达。结果中子照射后6h,IEC-6细胞凋亡率增加(P<0·01),应用rhIL-11可以降低细胞凋亡率(P<0·05)。IL-11Rα于正常IEC-6细胞膜和浆呈强阳性,中子照射后6h,IL-11Rα表达明显减少(P<0·01),24h恢复至正常水平(P<0·01),应用rhIL-11后IL-11Rα表达高于单纯照射组(P<0·05)。gp130于正常IEC-6细胞浆呈强阳性,中子照射后48h内呈进行性减少(P<0·05),应用rhIL-11后gp130表达于照射后24h恢复正常,48h降低但高于单纯照射组(P<0·05)。结论IL-11对IEC-6细胞中子辐射损伤起保护作用,其机制可能是通过上调其特异性结合受体亚基IL-11Rα和信号转导亚基gp130发挥作用。
Objective To investigate the effect of rhIL-11 on the expression of IL-11Rα and gp130 in IEC-6 cells after neutron irradiation. Methods The cultured IEC-6 cells were irradiated with 4.0 Gy neutron and rhIL-11 (100 ng / ml) was given 12 h before and immediately after irradiation. The IECs were detected by flow cytometry, immunohistochemistry, Western blot and image analysis -6 cells, the expression of IL-11Rα and gp130. Results 6h after neutron irradiation, the apoptosis rate of IEC-6 cells increased (P <0.01), and rhIL-11 could reduce the apoptosis rate (P <0.05). The expression of IL-11Rα in normal IEC-6 cell membrane and plasma was strongly positive. The expression of IL-11Rα at 6h after neutron irradiation was significantly decreased (P <0.01), and returned to normal level at 24h (P <0.01) IL-11Rα expression was higher than that of the control group (P <0.05). The expression of gp130 in normal IEC-6 cells was strongly positive and neutrons decreased progressively within 48 hours (P <0.05). After treated with rhIL-11, gp130 expression returned to normal at 24h and decreased at 48h Irradiation group (P <0.05). Conclusion IL-11 may play a protective role in neutron radiation injury in IEC-6 cells. The mechanism may be through up-regulating IL-11Rα, a receptor binding subunit, and gp130, a signaling subunit.