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①目的研究铅对人红细胞膜脂质及膜蛋白的作用，并观察尼克酰胺（ＮＭ）的抗铅作用。②方法用荧光偏振法与电子自旋共振自旋标记法，检测了铅处理组及对照组人红细胞膜的荧光偏振度（Ｐ）、微粘度（η）（ｎ＝４）及膜蛋白的电子自旋共振波谱中弱、强固定化组分峰值的比值ｈｗ／ｈｓ（ｎ＝３），结果经ｔ检验统计处理。③结果铅处理过人红细胞膜的荧光偏振度、微粘度及ｈｗ／ｈｓ比值均减小，与对照组比较差异均有显著性（ｔ＝３．１８８～９．１６５，Ｐ均＜０．０５）。尼克酰胺能减弱铅致人红细胞膜荧光偏振度、微粘度及ｈｗ／ｈｓ比值的减小（ｔ＝３．２２８～９．８０６，Ｐ均＜０．０５）。④结论铅可致红细胞膜脂双层疏水区的流动性增大，膜蛋白的构象发生变化。尼克酰胺能减弱铅对红细胞膜的作用
①Objective To study the effect of lead on human erythrocyte membrane lipid and membrane protein and to observe the anti-lead effect of nicotinamide (NM). Methods The fluorescence polarization (P), microviscosity (η) (n = 4), and electron of membrane protein were detected by fluorescence polarization and electron spin resonance spin labeling. In the spin resonance spectrum, the ratio of the peak values of weak and strong immobilized components, hw / hs (n = 3), was statistically analyzed by t-test. ③ Results The fluorescence polarization degree, microviscosity and hw / hs ratio of erythrocyte membrane in the lead treated group decreased significantly compared with the control group (t = 3.188-9.165, P <0.05 respectively) ). Nicotinamide attenuated the decrease of fluorescence polarization degree, microviscosity and hw / hs ratio of lead-induced human erythrocyte membrane (t = 3.228-9.806, P <0.05). ④ Conclusion lead can lead to red blood cell membrane lipid bilayer hydrophobicity increases, the conformation of the membrane protein changes. Nicotamide can reduce the role of lead on the erythrocyte membrane