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目的:建立HPLC法同时测定复方青蒿安乃近片中两成分的含量。方法:色谱柱为Waters Symmetry C18(250 mm×4.6 mm,5μm);流动相为甲醇-0.02 mol·L~(-1)磷酸二氢钾(25∶75,用磷酸调p H至3.5);流速为1.0 ml·min~(-1);检测波长为250 nm;柱温为30℃;进样量为20μl。结果:安乃近质量浓度在50.11~601.36μg·ml~(-1)(r=0.999 9)内,葛根素质量浓度在2.64~31.74μg·ml~(-1)(r=0.999 8)内与峰面积呈良好的线性关系。安乃近和葛根素的平均回收率分别为99.1%(RSD=0.7%,n=9)和99.9%(RSD=0.6%,n=9)。结论:该法简便准确、重复性好,可用于该制剂的质量控制。
Objective: To establish an HPLC method for the simultaneous determination of two components in the compound artemisiamphnadol. METHODS: The chromatographic column was Waters Symmetry C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.02 mol·L -1 potassium dihydrogen phosphate (25:75, pH = 3.5 with phosphoric acid) The flow rate was 1.0 ml · min -1. The detection wavelength was 250 nm. The column temperature was 30 ℃. The injection volume was 20 μl. Results: The concentration of metamizole in 50.11 ~ 601.36μg · ml ~ (-1) (r = 0.999 9), the concentration of puerarin in 2.64 ~ 31.74μg · ml ~ (-1) (r = 0.999 8) And the peak area showed a good linear relationship. The average recoveries of metamizole and puerarin were 99.1% (RSD = 0.7%, n = 9) and 99.9% (RSD = 0.6%, n = 9), respectively. Conclusion: The method is simple and accurate, reproducible, and can be used for the quality control of the preparation.