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目的:通过监测新生儿缺氧缺血性脑病(HIE)患儿血清中S-100蛋白含量的动态变化,建立对HIE患儿的早期科学诊断及预后评估技术指标。并探讨S-100蛋白浓度变化与病情的严重程度及转归之间的关系。方法:采用酶联免疫吸附试验、双抗体夹心法检测,对120例HIE有症状组患儿(HIE轻度症状组40例,HIE中度症状组40例,HIE重度症状组40例)和40例新生儿窒息组患儿与40例对照组健康新生儿血清S-100蛋白含量进行检测,并进行动态观察。结果:①HIE有症状组不同时期血清S-100蛋白含量均显著高于对照组(P<0.01);②新生儿窒息组不同时期的血清S-100蛋白含量也明显高于对照组(P<0.01);③HIE有症状组血清S-100蛋白含量明显高于新生儿窒息组(P<0.05)。结论:HIE有症状组患儿病情越重,其血清S-100蛋白含量则越高,提示血清S-100蛋白可作为判断HIE病情程度的客观依据和早期诊断以及预后评估HIE的主要指标。
OBJECTIVE: To establish an early scientific diagnosis and prognostic assessment of HIE children by monitoring the changes of serum S-100 protein levels in neonates with hypoxic-ischemic encephalopathy (HIE). And to explore the relationship between the change of S-100 protein concentration and the severity of the disease and the outcome. Methods: Enzyme-linked immunosorbent assay (ELISA) and double antibody sandwich method were used to detect the level of HIE in 120 HIE-symptomatic children (40 mild HIE, 40 HIE moderate, 40 severe HIE) and 40 Cases of neonatal asphyxia group and control group 40 healthy newborn serum S-100 protein levels were detected and dynamically observed. Results: ① The content of S-100 protein in HIE group was significantly higher than that in the control group (P <0.01); ② The content of S-100 protein in neonatal asphyxia group was also significantly higher than that in the control group (P <0.01) ); ③ HIE symptomatic group serum S-100 protein content was significantly higher than neonatal asphyxia group (P <0.05). CONCLUSIONS: The more serious the disease in children with symptomatic HIE, the higher the serum S-100 protein content, suggesting that serum S-100 protein can be used as an objective basis for judging the severity of HIE and early diagnosis and prognosis evaluation of HIE main indicators.