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目的:研究重度子痫前期患者母儿间HLA Ⅱ类抗原HLA-DRB1,-DQB1和-DPB1有无相容性或相斥性。方法:用多聚酶链反应-限制性片段长度多态性分析(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法检测31例重度子痫前期组母儿和34例正常妊娠母儿的HLA-DRB1,-DQB1和-DPB1的基因型和表型。计算母儿间不一致的HLA基因数和(或)抗原数,比较两组间的差异。结果:未发现重度子痫前期组母儿间存在明显的HLA-DRB1,-DQB1或-DPB1基因型或表型的显著相容或相斥。但是,重度子痫前期组母儿间HLA-Ⅱ类抗原基因型的相容性显著多于正常对照组,母儿间Ⅱ类抗原基因型完全相符的例数显著高于对照组(分别为4例和0例,P=0.046)。结论:母儿间HLA Ⅱ类抗原基因型相容性增加可能参与了重度子痫前期的发病。
OBJECTIVE: To investigate the compatibility or repulsion of HLA class II antigens HLA-DRB1, -DQB1 and -DPB1 in patients with severe preeclampsia. Methods: The HLA-B expression of 31 pregnant women with severe preeclampsia and 34 normal pregnant women were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) The genotypes and phenotypes of DRB1, -DQB1 and -DPB1. Calculate the number of HLA genes and / or antigens inconsistent between mother and child and compare the difference between the two groups. RESULTS: No significant compatibility or repulsion of HLA-DRB1, -DQB1 or -DPB1 genotypes or phenotypes was found between maternal and females with severe preeclampsia. However, the compatibility of HLA-Ⅱgenotypes between mother and child in severe preeclampsia group was significantly higher than that of normal control group, and the number of mother-to-child type Ⅱ antigen genotypes was significantly higher than that of control group (4 Cases and 0 cases, P = 0.046). Conclusion: The increased compatibility of maternal and neonatal HLA Ⅱ genotypes may be involved in the pathogenesis of severe preeclampsia.