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目的:探讨不同剂量芪苈强心胶囊对心衰模型大鼠非梗死区胶原蛋白分子表达的影响。方法:将通过结扎冠状动脉左前降支并饲养4周的56只心衰模型鼠随机分成4组:心衰对照组(MI-C)、转换酶抑制剂雷米普利治疗组(MI-R,10 mg/kg.d)、芪苈强心小剂量组(MI-S,0.25 g/kg.d)以及芪苈强心大剂量组(MI-L,1.0 g/kg.d)。同步药物干预4周后,ELISA法检测Ang II水平、RT-PCR检测非梗死区胶原-I mRNA。结果:血清中Ang II的浓度:与心力衰竭对照组比较,假手术组、雷米普利组、大剂量芪苈强心组和小剂量芪苈强心组均明显降低(P<0.05)。其中,大剂量芪苈强心组比雷米普利组明显减低,差异具显著性(P<0.05);而小剂量芪苈强心组与雷米普利组水平接近,差异无显著性(P<0.05)。非梗死区胶原-I mRNA的表达:与心衰对照组比较,假手术组、雷米普利组、大剂量芪苈强心组,小剂量芪苈强心组表达均下调,差别具显著性(P<0.05);大剂量芪苈强心组与雷米普利组接近,差别无显著性(P>0.05);小剂量芪苈强心组高于大剂量芪苈强心和雷米普利组,差别具有显著性(P<0.05)。结论:芪苈强心胶囊能够明显地减少心梗后心衰非梗死区胶原分子的合成,并具有明显的剂量依赖性。
Objective: To investigate the effect of Qili Qiangxin Capsule on the expression of collagen molecules in non-infarcted area in rats with heart failure. Methods: Fifty-six heart failure model rats were established by ligation of the left anterior descending coronary artery and rearing for 4 weeks: MI-C, MI-R, , 10 mg / kg.d), low dose Qiliqiangxin (MI-S, 0.25 g / kg.d), and high dose Qiliqiangxin (MI-L, 1.0 g / kg.d). After 4 weeks of concurrent drug intervention, Ang II level was detected by ELISA and collagen-I mRNA was detected by RT-PCR in non-infarcted area. Results: The concentration of Ang II in serum: Compared with heart failure control group, the sham operation group, ramipril group, high dose Qizhixiangxin group and low dose Qiqiangqiangxin group were significantly lower (P <0.05). Among them, the high-dose Qiliqiangxin group was significantly lower than the ramipril group, the difference was significant (P <0.05); low-dose Qiliqiangxin group and ramipril group level close, the difference was not significant P <0.05). Non-infarct collagen-I mRNA expression: Compared with the heart failure control group, sham operation group, ramipril group, high-dose Qiliqiangxin group, low-dose Qiliqiangxin group were downregulated, the difference was significant (P <0.05); high-dose Qiliqiangxin group and Ramipril group close, the difference was not significant (P> 0.05); low-dose Qiliqiangxin group was higher than high-dose Qiliqiangxin and Ramipu Lee group, the difference was significant (P <0.05). Conclusion: Qili Qiangxin Capsule can significantly reduce the non-infarction area after myocardial infarction collagen molecule synthesis, and has a clear dose-dependent manner.