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目的:分析乳癌患者转移淋巴结标本中T细胞受体(TCR)β链互补决定区3(CDR3)的谱型偏移及氨基酸序列特点。方法:选取5例乳癌患者转移淋巴结和2例乳腺反应性增生患者的淋巴结标本,应用RT-PCR扩增T细胞24个TCRβ链可变区(BV)亚家族,经基因扫描确定T细胞克隆性,对提示单、寡克隆性增生的TCR BV亚家族的PCR产物进行测序。结果:乳腺反应性增生患者淋巴结中T细胞均为多克隆性增生。乳癌患者转移淋巴结中T细胞呈单、寡克隆及多克隆性增生,克隆性增生T细胞中存在2~5个BV亚家族的谱型偏移;CDR3氨基酸序列分析证实单、寡克隆性增生T细胞的CDR3氨基酸序列呈现多样性的特点。结论:乳癌患者TCR BV亚家族取用存在谱型偏移,不同T细胞克隆的CDR3氨基酸序列不同。
OBJECTIVE: To analyze the spectral shift and amino acid sequence of TCR β3 complementarity determining region (CDR3) in metastatic lymph node specimens from patients with breast cancer. Methods: Lymph nodes were collected from 5 patients with metastatic lymph nodes and 2 patients with reactive hyperplasia of mammary carcinoma. 24 TCR β chain (BV) subfamilies of T cells were amplified by RT-PCR. The clonality of T cells , The PCR products of the TCR BV subfamily of hints and oligoclonal proliferation were sequenced. Results: T lymphocytes in hyperplasia of mammary glands were polyclonal hyperplasia. T lymphocytes in lymph node metastasis of breast cancer patients showed single, oligoclonal and polyclonal hyperplasia, and there were 2 to 5 BV subfamilies in clonal hyperplastic T cells. CDR3 amino acid sequence analysis confirmed single and oligoclonal hyperplasia T The CDR3 amino acid sequence of the cell is characterized by diversity. CONCLUSIONS: There is a spectral shift in access to the TCR BV subfamily of breast cancer patients, with different CDR3 amino acid sequences for different T cell clones.