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研究抑瘤素M(oncostatin M,OSM)对人胃癌细胞株MKN-28体外侵袭能力和环氧合酶-2(COX-2)表达的影响,探讨其在胃癌浸润转移中的作用及可能机制。运用Transwell小室法检测OSM对胃癌细胞迁移的影响;将靶向COX-2基因的小分子干扰RNA(siRNA)瞬时转染胃癌细胞,采用蛋白印迹法(western blot)和MTT法分析转染后胃癌细胞COX-2蛋白表达情况和细胞生长情况;荧光定量聚合酶链反应(RT-PCR)和western blot分别检测25 ng/ml的OSM对胃癌细胞COX-2 mRNA和蛋白表达的影响。研究发现,25 ng/ml OSM能促进胃癌细胞的迁移(P<0.05)。用针对COX-2的siRNA转染胃癌细胞后,COX-2蛋白表达降低,细胞生长变慢。经25 ng/ml OSM作用的胃癌细胞COX-2 mRNA及蛋白的表达升高(P<0.05)。结果提示,一定剂量的OSM可能通过诱导COX-2的表达,促进胃癌细胞侵袭和转移。
To investigate the effect of oncostatin M (OSM) on invasion and COX-2 expression of human gastric cancer cell line MKN-28 in vitro and to explore its possible role in invasion and metastasis of gastric cancer . Transwell chamber assay was used to detect the effect of OSM on gastric cancer cell migration. Small interfering RNA (siRNA) targeting COX-2 gene was transiently transfected into gastric cancer cells. Western blot and MTT were used to analyze the expression of gastric cancer The effect of 25 ng / ml OSM on the expression of COX-2 mRNA and protein in gastric cancer cells was detected by quantitative real-time polymerase chain reaction (RT-PCR) and western blot. The study found that 25 ng / ml OSM can promote gastric cancer cell migration (P <0.05). After transfection of gastric cancer cells with siRNA against COX-2, COX-2 protein expression decreased and cell growth slowed down. The expression of COX-2 mRNA and protein in gastric cancer cells treated with 25 ng / ml OSM increased (P <0.05). The results suggest that a certain dose of OSM may promote gastric cancer cell invasion and metastasis by inducing COX-2 expression.