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目的研究虾青素对四氯化碳(CCl4)所致小鼠急性化学肝损伤的保护作用。方法雄性昆明种小鼠60只,随机分为正常对照组、急性化学性肝损伤模型组、联苯双酯(15 mg/kg)阳性对照组以及虾青素10,15,20 mg/kg剂量组,共6组。测定各组小鼠肝脏系数、血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性与丙二醛(MDA)含量;测定肝组织中SOD、GSH-Px活性、MDA含量以及组织病理系数。结果虾青素3个剂量组小鼠血清SOD由低到高依次为(110.18±14.28),(138.09±17.81),(170.19±20.20)U/(mg pro);肝组织SOD由低到高依次为(141.52±28.29),(172.20±29.24),(200.12±33.42)U/(mg pro);与模型组血清(74.52±14.40)U/(mg pro)、肝组织(102.29±34.29)U/(mg pro)比较均明显升高(P<0.01)。虾青素3个剂量组小鼠血清GSH-Px活性由低到高次为(63.30±11.01),(72.40±10.77),(90.17±11.29)U/(mg pro);肝组织GSH-Px活性由低到高依次为(112.29±25.41),(142.96±24.13),(170.38±26.34)U/(mg pro);与模型组血清(60.13±10.28)U/(mg pro)、肝组织(110.69±24.26)U/(mg pro)比较均明显升高(P<0.01)。虾青素3个剂量组小鼠血清ALT由低到高依次为(99.29±5.27),(83.50±5.12),(68.12±5.12)U/L;血清AST活性由低到高依次为(80.49±4.28),(64.20±4.10),(52.21±4.42)U/L,与模型组血清ALT(108.02±15.06)U/L、血清AST活性(97.28±12.28)U/L比较明显降低;血清与肝组织MDA含量明显降低,差异均有统计学意义(P<0.01),并能不同程度地改善肝脏病理组织损伤。结论虾青素对CCl4所致急性化学性肝损伤具有预防性保护作用。
Objective To study the protective effect of astaxanthin on acute chemical liver injury induced by carbon tetrachloride (CCl4) in mice. Methods Sixty male Kunming mice were randomly divided into normal control group, acute chemical liver injury model group, bifendate (15 mg / kg) positive control group and astaxanthin 10,15,20 mg / kg dose Group, a total of 6 groups. The hepatic coefficient, serum ALT, AST, SOD and GSH of the mice in each group were measured. -Px) activity and malondialdehyde (MDA) content. The activities of SOD, GSH-Px, MDA and histopathological parameters in liver tissue were determined. Results The levels of SOD in the asthma group were (110.18 ± 14.28), (138.09 ± 17.81) and (170.19 ± 20.20) U / (mg pro), respectively. The SOD in liver tissue from low to high was (141.52 ± 28.29), (172.20 ± 29.24) and (200.12 ± 33.42) U / mg pro, respectively. Compared with model group serum 74.52 ± 14.40 U / mg pro and 102.29 ± 34.29 U / (mg pro) were significantly higher (P <0.01). The activities of GSH-Px in asthma group were (63.30 ± 11.01), (72.40 ± 10.77) and (90.17 ± 11.29) U / (mg pro), respectively. The activities of GSH-Px (112.29 ± 25.41), (142.96 ± 24.13) and (170.38 ± 26.34) U progesterone (mg pro) in model group and 60.13 ± 10.28 mg pro and 110.69 ± 24.26) U / (mg pro) were significantly higher (P <0.01). The levels of serum ALT from low to high were (99.29 ± 5.27), (83.50 ± 5.12) and (68.12 ± 5.12) U / L, respectively. The serum AST activities from low to high were (80.49 ± 4.28), (64.20 ± 4.10) and (52.21 ± 4.42) U / L, respectively. Compared with the model group, serum ALT (108.02 ± 15.06) U / L and serum AST activity (97.28 ± 12.28) U / Tissue MDA content was significantly lower, the difference was statistically significant (P <0.01), and to varying degrees, improve liver pathological tissue injury. Conclusion Astaxanthin has a protective effect against CCl4-induced acute chemical liver injury.