以菠萝皮渣为原料,研究纳米TiO2吸附结合超滤、冷冻干燥技术制备菠萝蛋白酶粉的方法。结果表明:在温度15℃条件下,以粗酶液质量3.5%的纳米TiO2吸附30min,酶吸附率达99.1%;采用0.4mol/L、pH6.0的柠檬酸缓冲液洗脱,将洗脱液用截留分子质量50kD的超滤膜超滤,流出液用截留分子质量10kD的超滤膜超滤,浓缩液经冷冻干燥得到比活力为2.8×106U/g的菠萝蛋白酶粉。 Using pineapple skin residue as raw material, the method of preparing bromelain powder by nano-TiO2 adsorption combined with ultrafiltration and freeze-drying technology was studied. The results showed that the adsorption rate of nano-TiO2 with the mass fraction of 3.5% of crude enzyme solution was 99.1% for 30 min under the conditions of 15 ℃, and eluted with 0.4mol / L citric acid buffer (pH 6.0) The liquid was ultrafiltrated by a ultrafiltration membrane with molecular weight cut-off of 50kD. The effluent was ultrafiltrated by a ultrafiltration membrane with a molecular weight cut-off of 10kD. The concentrate was lyophilized to obtain bromelain powder with a specific activity of 2.8 × 10 6 U / g.