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目的探讨重组人白细胞介素24(rhIL-24)联合顺铂(DDP)诱导人肺腺癌A549细胞凋亡相关基因的变化。方法用160 ng/m L rhIL-24、3μg/m L DDP及160 ng/m L rhIL-24联合3μg/m L DDP处理A549细胞,应用Genome LabTMGe XP多基因遗传表达分析系统检测凋亡相关基因Bax、Bcl-2、存活蛋白(survivin)、caspase-3、视网膜母细胞瘤基因(Rb)、p53的mRNA表达水平。结果 rhIL-24蛋白处理的A549细胞上调Bax、caspase-3、Rb的mRNA水平,下调Bcl-2、survivin的mRNA水平,但p53mRNA变化无规律,rhIL-24联合DDP组对A549细胞上述基因表达的影响更明显。结论 rhIL-24蛋白可通过上调Bax、抑癌基因Rb,下调Bcl-2、survivin,激活caspase-3而诱导人肺腺癌A549细胞凋亡。
Objective To investigate the changes of apoptosis related genes in human lung adenocarcinoma A549 cells induced by recombinant human interleukin 24 (rhIL-24) and cisplatin (DDP). Methods A549 cells were treated with 160 ng / mL rhIL-24, 3 μg / mL LDP and 160 ng / mL rhIL-24 plus 3 μg / mL LDP. Genome LabTMGe XP polygene analysis system was used to detect apoptosis-related genes Bax, Bcl-2, survivin, caspase-3, retinoblastoma gene (Rb) and p53. Results The mRNA and protein expressions of Bax, caspase-3 and Rb were upregulated in rhIL-24-treated A549 cells. The mRNA levels of Bcl-2 and survivin were down-regulated in rhIL-24-treated A549 cells. The impact is more obvious. Conclusion rhIL-24 protein can induce apoptosis of human lung adenocarcinoma A549 cells by up-regulating Bax, tumor suppressor gene Rb, down-regulating Bcl-2, survivin and activating caspase-3.