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本研究通过室内直接吸收法测定了四环素、金霉素、诺氟沙星和恩诺沙星4种典型抗生素对土壤微生物呼吸作用的影响。结果表明,4种抗生素对土壤微生物呼吸的影响存在一定差异。四环素在整个处理过程中对土壤呼吸的影响以抑制作用为主;金霉素在处理第1d和7d均有激活作用,而且激活率最高可达到51.23%,但是在其他处理时间以抑制作用为主;诺氟沙星在整个处理过程中,浓度为0.2mg·kg-1和1mg·kg-1对土壤微生物呼吸都有一定的抑制作用,浓度为5、25、50mg·kg-1处理均为激活作用,激活作用随着处理浓度的增加而升高。恩诺沙星在整个处理过程中,所有处理对呼吸的影响变化趋势为先激活后抑制。总体来说,到处理第15d,所有抗生素处理的微生物呼吸作用基本恢复到对照水平,说明在本试验浓度处理下,微生物对这4种抗生素能够产生一定的适应性。
In this study, the effects of four typical antibiotics, tetracycline, chlortetracycline, norfloxacin and enrofloxacin, on the respiration of soil microorganisms were determined by direct indoor absorption. The results showed that the four kinds of antibiotics on the impact of soil microbial respiration there are some differences. Tetracycline had the most inhibitory effect on soil respiration during the whole treatment. Chlortetracycline had the activation effect on the 1st day and the 7th day, and the highest activation rate reached 51.23%. However, it was mainly inhibited by other treatments . Norfloxacin at concentrations of 0.2 mg · kg-1 and 1 mg · kg-1 all inhibited the respiration of soil microorganisms at all concentrations for 5, 25 and 50 mg · kg-1 treatments Activation, activation increased with the treatment concentration. Enrofloxacin throughout the treatment process, the impact of all treatments on the respiratory changes in the first inhibition after activation. Overall, by the 15th day of treatment, the respiration of all the antibiotic-treated microorganisms basically returned to the control level, indicating that the microorganisms can adapt to these four antibiotics under the concentration test of this test.