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本文描述一种对Sj的小鼠IgE单克隆抗体,在感染早期被动转移该抗体对小鼠有部分的而明显的保护作用。材料与方法:用机械方法制备童虫,然后在5％CO_2、37℃下孵育3小时。按Chaffee法略加改良制备Sj、Sm及肝片形吸虫,卫氏并殖吸虫,旋毛虫抗原。在制备Sj成虫抗原时,单性雄虫与配对的成虫分开制备。转化的Sj童虫抗原亦分别制备。慢性感染血清为感染20～30条Sj尾蚴8～12周后的小鼠血清。McAb的制备:经Sj成虫抗原免疫对Sj产生高水平IgE抗体的C3H/He小鼠的脾细胞与NS-1骨髓瘤细胞融合,以被动皮肤过敏反应(PCA)筛选阳性杂交瘤,经克隆化获得IgE单克隆抗体。
This article describes a mouse monoclonal anti-Sj IgE monoclonal antibody that is passively transferred early in the infection to provide some and significant protection in mice. MATERIALS AND METHODS: Schistosomas were prepared mechanically and then incubated for 3 hours at 37 ° C with 5% CO 2. According to Chaffee Law slightly modified Sj, Sm and liver Fasciola, Clostridium parasite, Trichinella antigen. In the preparation of Sj adult antigens, the unipod is prepared separately from the paired adults. The transformed Sj sperm antigens are also separately prepared. Chronic infection serum is 20 to 30 Sj cercariae 8 to 12 weeks after the mouse serum. Preparation of McAbs: Spleen cells of C3H / He mice immunized with Sj adult antigens to produce high levels of IgE antibodies against Sj were fused with NS-1 myeloma cells and positive hybridomas were screened by passive cutaneous anaphylaxis (PCA) IgE monoclonal antibody was obtained.