目的:观察苓芍枣仁方对膀胱过度活动症（overactive bladder, OAB）模型大鼠尿动力学及机械敏感离子通道Piezo1表达的影响。方法:将30只SPF级雌性SD大鼠按随机数字表法分为空白组、模型组、托特罗定组及苓芍枣仁方低、高剂量组，每组6只。采用环磷酰胺腹腔注射法建立OAB大鼠模型。造模成功后，托特罗定组灌胃酒石酸托特罗定混悬液0.36 mg/kg，苓芍枣仁方低、高剂量组灌胃1.59、3.18 g/kg苓芍枣仁免煎颗粒，空白组与模型组灌胃等体积的蒸馏水，1次/d，连续灌胃14 d。末次给药后检测各组大鼠尿失禁时膀胱容量、尿失禁时最大膀胱压力，采用HE染色观察各组大鼠膀胱组织病理形态学改变，采用免疫组化法及Western blot法检测膀胱组织Piezo1蛋白表达，采用qPCR法检测膀胱组织Piezo1 mRNA表达。结果:与空白组比较，模型组大鼠体重明显减轻，膀胱容量、最大膀胱压均明显降低(n P<0.01) ；HE染色显示，模型组尿路上皮增生，上皮细胞变性、坏死、脱落，间质大量炎性细胞浸润，血管增生，血管壁增厚，黏膜平滑肌增生，排列紊乱；膀胱组织Piezo1 mRNA及蛋白表达上调(n P< 0.05或n P<0.01)。与模型组比较，托特罗定组及苓芍枣仁方高剂量组大鼠体重[(244.83±6.05)g、(233.33±11.76)g比(219.00±9.70)g]显著增加(n P<0.01)，托特罗定组及苓芍枣仁方低、高剂量组大鼠膀胱容量[(0.93±0.31)ml、(1.17±0.17)ml、(1.21±0.23)ml比(0.50±0.16)ml]、最大膀胱压力[(42.00±3.03)cmHn 2O、(45.83±7.19)cmHn 2O、(46.83±8.23)cmHn 2O比(30.50±5.47)cmHn 2O]明显升高(n P<0.01)；膀胱组织上皮增生及变性程度、间质炎性细胞浸润程度、血管增生及黏膜平滑肌增生程度均明显减轻，托特罗定组及苓芍枣仁方低、高剂量组Piezo1 mRNA[(1.50±0.04)、(2.05±0.08)、(1.44±0.10）比（2.56±0.11)]及蛋白表达降低(n P<0.01)。n 结论:苓芍枣仁方可增加膀胱过度活动症大鼠逼尿肌活动过度引起的尿失禁的膀胱容量和尿失禁时最大膀胱压，其缓解尿频尿急症状可能与降低机械敏感离子通道Piezo1表达有关。“,”Objective:To observe the effect of n Lingshao-Zaoren Decoction on urodynamics and the expression of Piezo1 if overactive bladder (OAB) rats.n Methods:Thirty SPF grade female SD rats were randomly divided into blank group, model group, Tolterodine control group, low-dose and high-dose n Lingshao-Zaoren Decoction groups, with 6 rats in each group. The OAB rats were modeled by intraperitoneal injection of Cyclophosphamide. After the successful modeling, Tolterodine control group was given 0.36 mg/kg Tolterodine tartrate, the low-dose and high-dose n Lingshao-Zaoren Decoction groups were given 1.59 and 3.18 g/kg n Lingshao-Zaoren Mianjian granules by gavage, the blank group and model group were given the same amount of distilled water, once a day for 14 days. After 14 days, the urodynamics of rats in each group were detected. The bladder volume and maximum bladder pressure were observed respectively. The pathological changes of bladder tissue were observed by HE staining. The expression of Piezo1 protein in bladder tissue was detected by immunohistochemistry and Western blot. The expression of Piezo1 mRNA in bladder tissue was detected by qPCR.n Results:Compared with the blank group, the body weight, bladder volume and maximum bladder pressure of the model group were significantly reduced (n P<0.01). HE staining result showed that the model group had hyperplasia of urinary tract epithelium, degeneration, necrosis and abscission of epithelial cells, infiltration of a large number of inflammatory cells in stroma, vascular proliferation, thickening of vascular wall, hyperplasia of mucosal smooth muscle, disorder of arrangement, and significant up regulation of Piezo1 protein expression (n P<0.01). Compared with the model group, the weight [(244.83±6.05) g, (233.33±11.76) gn vs. (219.00±9.70) g] of rats in the Tolterodine control group and high-dose group of n Lingshao-Zaoren Decoction significantly increased (n P<0.01), and the bladder volume [(0.93±0.31) ml, (1.17±0.17) ml, (1.21±0.23) mln vs. (0.50±0.16) ml] and maximum bladder pressure [(42.00±3.03) cmHn 2O, (45.83±7.19) cmHn 2O, (46.83±8.23) cmHn 2O n vs. (30.50±5.47) cmHn 2O] of rats in the Tolterodine control group, low-dose and high-dose n Lingshao-Zaoren Decoction groups were significantly increased (n P<0.01); the bladder epithelial hyperplasia and degeneration degree, interstitial inflammatory cell infiltration degree and vascular hyperplasia degree of rats in the Tolterodine control group, low-dose and high-dosen Lingshao-Zaoren Decoction groups significantly increased. The expression of Piezo1 mRNA (1.50±0.04, 2.05±0.08, 1.44±0.10 n vs. 2.56±0.11) and protein in the Tolterodine control group, low-dose and high-dose n Lingshao-Zaoren Decoction groups were significantly decreased (n P<0.01).n Conclusion:Lingshao-Zaoren Decoction can increase the bladder volume and maximum bladder pressure of urinary incontinence caused by detrusor overactivity in rats with overactive bladder, which may be related to reduction of Piezo1 expression.n