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对松浦镜鲤口灌甲砜霉素,分别设对照组、50 mg/kg(1倍正常用药量,推荐剂量组)、150 mg/kg(3倍正常用药量,中等剂量组)及250 mg/kg(5倍正常用药量,毒性剂量组),连续口灌给药15 d,每天1次,于停药24 h后,自实验鱼的肝、脾、肾、肠和鳃采取新鲜组织,浸泡于波恩氏液中,待进行石蜡切片制作。结果显示,与对照组相比,松浦镜鲤肝、脾和肾在1倍给药剂量时,出现大量深染的炎性细胞,随着给药剂量增加,组织结构遭到破坏程度加重;3倍正常用药量时,肝、脾和肾出现细胞空泡化现象,肾小管管腔界限不清;在5倍正常用药量时,各组织空泡化现象加重,肝和脾出现细胞减少,细胞核溶解。对肠组织而言,1倍给药组与对照组无差异;3倍正常用药量时,肠绒膜出现孔隙;5倍正常用药量时,少量肠绒毛结构不完整,出现脱落现象。鳃组织与对照组相比,给药组出现鳃丝肿胀现象,随给药剂量加大,鳃丝肿胀加重。研究表明,在将甲砜霉素应用于松浦镜鲤的疾病防控时,应严格遵照药物的使用剂量进行给药,如果因病情原因或使用错误而造成使用剂量加大时,应对松浦镜鲤肝、肾等组织进行检查,来确定药物对鱼体的损伤程度。[中国渔业质量与标准,2014,4(3):54-59]
Thiamphenicol was injected into the carp mouth at Songpu Hospital. The rats in the control group were treated with 50 mg / kg (1 times normal dosage, recommended dosage), 150 mg / kg (3 times normal dosage, medium dosage) and 250 mg / kg (5 times the normal dosage, toxic dose group), continuous oral administration of 15 d, 1 day, after stopping 24 h, from the experimental fish liver, spleen, kidney, intestine and gills to take fresh tissue, Soak in Bonn’s solution, to be paraffin sections. The results showed that compared with the control group, a large number of deep-stained inflammatory cells appeared in the liver, spleen and kidney of the liver, spleen and kidney of the pine carp Cyprinus carpio at a dose of 1 time. The damage of the tissue structure was aggravated with the increase of the dosage. When normal dosage was used, cell vacuolization occurred in the liver, spleen and kidney, and the lumen of the renal tubule was unclear. At 5 times normal dose, the vacuolization of each tissue increased, the number of cells in the liver and spleen decreased, Dissolved. For intestinal tissue, there was no difference between the 1-times administration group and the control group. When the normal dosage was 3 times, there was voids in the intestinal villi. When 5-times normal dosage, a small amount of intestinal villus structure was incomplete and shedding occurred. Gill tissue compared with the control group, the administration group appeared gill filament swelling phenomenon, with the dose increased, gill filament swelling increased. Studies have shown that when applying thiamphenicol to the disease prevention and control of the pine carp mirror carp, it should be strictly administered in accordance with the dose of the drug. If the dosage is increased due to the cause of illness or the wrong use, Liver, kidney and other organizations to check to determine the extent of drug damage to the fish body. [Chinese Fisheries Quality and Standards, 2014,4 (3): 54-59]