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目的探讨丙酮酸钠对新生鼠低血糖脑损伤的神经保护作用。方法 36只新生Wistar大鼠随机分为胰岛素处理P组(INS-P组,n=12)、胰岛素+丙酮酸钠处理组(INS-PP组,n=12)和对照组(n=12)。在出生后第2、4、6天分别采用皮下注射胰岛素(15 U/kg)诱导新生鼠低血糖;终止低血糖时INS-P组和INS-PP组分别皮下注射50%葡萄糖0.01 m L和50%葡萄糖0.01 m L+丙酮酸钠500 mg/kg;第3次低血糖处理后1天三组大鼠各取6只取脑组织标本,并采用Fluoro-Jade B(FJB)染色法进行死亡神经元的计数。低血糖损伤6周后,三组各6只大鼠用M orris水迷宫实验检测大鼠的空间学习和记忆能力。结果 INS-PP组与INS-P组相比,变性神经元计数为少,出生后6周INS-PP组大鼠的空间认知能力优于INS-P组,表现在INS-PP组大鼠找到平台的潜伏期短,在池中游泳距离短,在平台所在象限的逗留时间长,穿过平台位置的次数多。结论丙酮酸钠对胰岛素诱导的新生鼠低血糖脑损伤具有保护作用。丙酮酸钠给药可能是防止新生儿低血糖脑损伤的有效干预手段。
Objective To investigate the neuroprotective effects of sodium pyruvate on neonatal hypoglycemic brain injury. Methods Thirty-six newborn Wistar rats were randomly divided into 2 groups: insulin-treated P group (INS-P group, n = 12), insulin plus sodium pyruvate treatment group (n = 12) and control group . Neonatal hypoglycemia was induced by subcutaneous injection of insulin (15 U / kg) on days 2, 4 and 6, respectively. INS-P and INS-PP groups were subcutaneously injected with 50% glucose 0.01 m L and 50% glucose 0.01 m L + sodium pyruvate 500 mg / kg; 6 days after the third hypoglycemia treatment, 6 rats in each group were sacrificed and the brain tissues were harvested by Fluoro-Jade B (FJB) staining Yuan count. Six weeks after hypoglycemic injury, six rats in each group were tested for spatial learning and memory using Mris water maze test. Results The number of degenerative neurons in INS-PP group was less than that in INS-P group. The spatial cognitive ability of INS-PP group was better than that of INS-P group at 6 weeks after birth in INS-PP group Find the platform of incubation period is short, swimming in the pool is short, the platform where the quadrant of the long stay, the number of times through the platform position. Conclusion Sodium pyruvate has a protective effect on neonatal hypoglycemic brain injury induced by insulin. Sodium pyruvate administration may be an effective intervention to prevent neonatal hypoglycemic brain damage.