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各种测定蛋白酶活力方法已有系统综述。Guven等曾报道一种测定胰酶中蛋白酶活力的方法。底物血红蛋白经酶反应释放出色氨酸,后者对二甲氨基苯甲醛作用显色。本文改变了原法的供试品溶剂、酶反应温度和显色条件。精密量取供试品溶液(溶剂同中国药典1985年版,每1ml含蛋白酶活力约0.4单位)1.0ml置试管中,在37℃水浴保温10min,精密加入37℃预热的6%血红蛋白溶液(pH7.5,含36%尿素)5.0ml摇匀,立即置37±0.5℃水浴中准确反应30min,精密加入5%
Various methods for assaying protease activity have been systematically reviewed. Guven et al. Reported a method for determining protease activity in trypsin. Substrate hemoglobin by enzyme reaction to release tryptophan, the latter of the role of dimethylaminobenzaldehyde color. This article changed the original method for the test solvent, enzyme reaction temperature and color conditions. The precise amount of the test solution (solvent with the Chinese Pharmacopoeia 1985 edition, per 1ml protease activity of about 0.4 units) 1.0ml test tube, 37 ℃ water bath incubated 10min, precision by adding 37 ℃ preheated 6% hemoglobin solution (pH7 .5, containing 36% urea) 5.0ml shake, immediately set 37 ± 0.5 ℃ water bath accurate response 30min, precision by adding 5%