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抗性/耐受种群的适合度代价和中肠蛋白酶活性研究是了解昆虫抗性进化、抗性产生的生理生化基础及抗性治理的重要内容。本研究以室内继代汰选的具有相同遗传背景的Cry1Ac耐受和敏感品系为研究对象,系统研究了两品系的适合度和中肠蛋白酶活性差异。研究结果表明,与敏感品系相比,耐受品系幼虫的存活率显著下降,且耐受种群不同日龄幼虫体重均低于敏感种群,20日龄时达显著差异;但两品系不同虫态的发育速度、蛹重、化蛹率、羽化率和成虫繁殖力均没有显著差异。中肠蛋白酶活性研究表明,耐受种群的类胰蛋白酶活性显著低于敏感种群,但两种群的总蛋白酶和类胰凝乳蛋白酶活性没有显著差异。该研究对于识别蛋白酶介导的Bt抗性机制和昆虫Bt抗性治理均具有重要意义。
The study of the fitness cost and midgut protease activity of resistant / tolerant populations is an important part of understanding the physiological and biochemical basis and resistance management of insect resistance evolution and resistance. In this study, CrylAc tolerant and susceptible lines with the same genetic background, which were selected in the laboratory, were selected as the research object, and the differences between the two strains’ fitness and the midgut protease activity were systematically studied. The results showed that compared with the susceptible lines, the survival rate of tolerant line larvae significantly decreased, and the tolerant population larvae of different day-old larvae weight were lower than the sensitive population, 20-day-old significant differences; but the two strains of different insect states There was no significant difference in developmental speed, pupal weight, pupation rate, emergence rate and adult fecundity. The study of midgut protease activity showed that the tryptase activity of the tolerant population was significantly lower than that of the susceptible population, but the total protease and chymotrypsin activities of the two populations showed no significant difference. This study is of great importance for the identification of protease-mediated Bt resistance mechanisms and insect Bt resistance management.