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目的探讨溃疡性结肠炎(UC))患者糖基化终末产物受体(RAGE)的表达、血清可溶性糖基化终末产物受体(sRAGE)水平及其临床意义。方法 2012年3月至2014年3月收治的36例UC患者为UC组(活动期14例,缓解期22例),20例健康体检者为健康对照组,20例结肠镜检查肠黏膜正常者为正常对照组,20例普通结肠炎肠镜检查肠黏膜正常者为疾病对照组。采用酶联免疫吸附法(ELISA)检测各组血清sRAGE、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平,免疫组化染色检测结肠黏膜RAGE表达。结果 UC组患者血清sRAGE、CRP、TNF-α水平均高于疾病对照组和健康对照组(P均<0.05),活动期患者的血清sRAGE、CRP水平均高于缓解期(P均<0.05);各组血清中sRAGE水平和CRP、TNF-α水平呈正相关关系(r=0.4936,0.5984;P均<0.05)。UC组病变区的RAGE表达阳性率(86.1%)明显高于正常区(50.0%)及疾病对照组(55.0%)、正常对照组(45.0%),差异均有统计学意义(P均<0.05),但UC组正常区、疾病对照组、正常对照组的结肠黏膜RAGE表达阳性率之间差异均无统计学意义(P均>0.05)。结论 RAGE及sRAGE可能参与了UC的发病,是UC独特的免疫炎症性发病特点的反映。
Objective To investigate the expression of receptor for advanced glycation end products (RAGE) and serum soluble glycosylation end product receptor (sRAGE) in patients with ulcerative colitis (UC) and its clinical significance. Methods Thirty-six patients with UC who were admitted to our hospital from March 2012 to March 2014 were selected as UC group (active stage and remission period of 22 cases), 20 healthy subjects were healthy controls, 20 cases of colonoscopy were normal intestinal mucosa For the normal control group, 20 cases of normal colitis colonoscopy intestinal mucosa were normal control group. Serum levels of sRAGE, C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA) Results Serum levels of sRAGE, CRP and TNF-α in patients with UC were significantly higher than those in controls and healthy controls (all P <0.05). Serum levels of sRAGE and CRP in active patients were significantly higher than those in patients with remission (all P <0.05) The level of sRAGE in serum of each group was positively correlated with CRP and TNF-α (r = 0.4936, 0.5984, P <0.05). The positive rate of RAGE expression in the diseased area of UC group was significantly higher than that in normal area (50.0%) and disease control group (55.0%) and normal control group (45.0%) (P <0.05) ). However, there was no significant difference in the positive rates of RAGE between colonic mucosa in normal group, disease control group and normal control group (all P> 0.05). Conclusions RAGE and sRAGE may be involved in the pathogenesis of UC, which is a reflection of the unique immunologic and inflammatory features of UC.