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在试验的33个非洲紫罗兰(Saintpaulia ionantha Wendl.)品种中,有31个品种的叶片和叶柄外植体可在 MS+BA 0.1nag/l+NAA 0.1mg/l 琼脂培养基上再生出有少量根的小植株。按常规的试管繁殖法,需将这些小苗转移到无激素的 MS 琼脂培养基上作第二步培养,以便成长为具较多根的大试管苗,再移出栽种入育苗盆。本研究改用 MS+人参粉250mg/l液体培养基在摇床作第二步培养,提出了一个高效快速的非洲紫罗兰新的试管繁殖法,用此方法可在较短时间内得到比插叶繁殖法多470—780倍、比目前试管培养法多200%的大试管苗。本文还报道了器官发生过程的扫描电子显微镜研究结果。
Among 33 tested varieties of Saintpaulia ionantha Wendl., 31 cultivars of leaf and petiole explants were regenerated with a small amount of MS + BA 0.1 nag / l + NAA 0.1 mg / l agar medium Roots of small plant. According to the conventional test tube propagation method, these seedlings are transferred to hormone-free MS agar medium for second-step culture so as to grow into large test-tube seedlings with more roots and then removed for planting in nursery pots. In this study, MS + ginseng powder 250mg / l liquid medium instead of shaker for the second step culture, proposed a high efficient and rapid new African Violets test tube propagation method, with this method can be obtained in a shorter time than leaf propagation Fado 470-780 times, 200% more than the current test tube culture test tube plantlets. This article also reports the results of scanning electron microscopy of organogenesis.