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以往曾用3-(3-甲氧基苯蒽酮)荧光探针研究支气管哮喘患者淋巴细胞磷脂层的状态,结果砭实,3荧光探针强度明显降低,表明淋巴细胞膜的病理改变。用金霉素荧光探针研究证明钙离子通过生物膜或人造膜的转运,即金霉素荧光探针能正确地反映钙离子的转运动力学,还利用金霉素荧光探针研究了致敏淋巴细胞对变应原的反应,并确定这种反应对支气管哮喘患者的诊断价值。作者观察了79例患者(男41人,女38人),年龄18～46岁。其中支气管哮喘68例,过敏性鼻窦病和荨麻疹各5例,血管神经性水肿1例。以12例18～36岁的健康成年人作为对照组。用淋巴细胞分离液从外周血中分离出淋巴细胞,用金霉素染色,然后用细胞荧光仪
In the past, 3- (3-methoxybenzanthrone) fluorescent probe was used to study the status of lymphocyte phospholipid in patients with bronchial asthma. The results showed that the intensity of 3 fluorescent probe was significantly reduced, indicating the pathological changes of lymphocyte membrane. Fluorescent probe with chlortetracycline to prove that calcium ions through the biofilm or artificial membrane transport, that chlortetracycline fluorescent probe can correctly reflect the calcium transport kinetics, but also the use of aureomycin fluorescent probe sensitized lymph nodes Cells respond to allergens and determine the diagnostic value of this response in patients with bronchial asthma. The authors observed 79 patients (41 males and 38 females) aged 18-46 years. Including bronchial asthma in 68 cases, allergic sinusitis and urticaria in 5 cases, 1 case of angioneurotic edema. Twelve healthy adults aged 18-36 years served as the control group. Lymphocytes were isolated from peripheral blood by lymphocyte separation liquid, stained with chlortetracycline and stained with a cytofluorometer