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建立大鼠不同组织(脑、心、肺、肝、脾、小肠、肾、脂肪和肌肉)中s-油酰丙醇胺含量的测定方法,并研究s-油酰丙醇胺在大鼠体内的组织分布情况。s-油酰丙醇胺提取采用液-液萃取,以10-十一烯酰乙醇胺为内标。SD雄性大鼠灌胃给予s-油酰丙醇胺(50 mg.kg-1)后,于4个时间点(各时间点5只大鼠)处死大鼠后,取相应组织匀浆处理,通过LC-MS/MS法测定其各脏器或组织中浓度。通过多反应检测模式测得定量下限1μg.L-1,各组织匀浆中s-油酰丙醇胺在1～2×104μg.L-1内线性良好(r≥0.999 0),方法经验证符合生物样品分析要求。灌胃给药后药物浓度最高的组织为小肠和心脏,达峰时间分别为15和90 min。该法操作简便、灵敏度高,适用于s-油酰丙醇胺在大鼠体内的组织分布研究。
To establish a method for the determination of s-oleoylpropanolamine in different tissues (brain, heart, lung, liver, spleen, small intestine, kidney, fat and muscle) in rats and to study the effect of s- The organization of the distribution. s-Oleoylpropanolamine extraction using liquid-liquid extraction, 10-undecylenoylethanolamine as an internal standard. SD male rats were intragastrically given s-oleoyl propanolamine (50 mg.kg-1), at 4 time points (5 rats at each time point) after the rats were sacrificed, the corresponding tissue homogenate, The concentration in each organ or tissue was determined by LC-MS / MS. The limit of quantitation of 1μg.L-1 was determined by multiple reaction assay. The s-oleoylpropanolamine in each tissue homogenized well in 1 ~ 2 × 104μg.L-1 (r≥0.999 0) Meet the biological sample analysis requirements. Gavage after administration of the highest drug concentration in the tissue of small intestine and heart, the peak time was 15 and 90 min. The method is simple, high sensitivity, suitable for s-Oleoyl propanolamine in the distribution of tissue in rats.