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目的探讨血液标本的放置时间和方式对多项生化指标检测结果的影响。方法抽取101份病人血液标本3管/人，分别按三种不同方式处置：A组标本抽血后30m i n内离心分离血清进行多项生化指标检测；B组标本30m i n内离心但不去盖,放于4℃冰箱内存放,24h后检测标本的生化指标；C组标本不离心直接存放于4℃冰箱内,24h后离心检测标本的生化指标。根据本实验室开展的生化项目,本研究选择了ALT、AST、ALP、GGT、TP、ALB、AFU、CK等36项生化指标为研究对象。结果我们将A组结果为基准，与A组结果比较,B组的AST、LDH、HBDH、CKMB、Zn增高, ALP、GGT、TP、ALB、AFU、T-BIL、D-BIL、CRE、UA、Glu、Cys-C、TG、HDL-C、ApoA、RF、CRP、AMS下降，有统计学意义(P＜0.05)。C组和A组结果比较，AST、BUN 、HBDH、CKMB、LDH 、HBDH、CK、CKMB、Mg、Zn增高,ALP、GGT、ALB、T-BIL、D-BIL、CRE、Glu、CHO、ApoA、ApoB、RF、CA、AMS下降，有统计学意义(P＜0.05)。C组和B组结果比较，AST、TP、BUN、Cys-C、LDH、HBDH、CRP、Zn增高,ALP、T-BIL、D-BIL、CRE、Glu、ApoB、ASO、CA下降，有统计学意义(P＜0.05)。结论本实验证明血标本离体后会因不同的放置时间和方式而引起生化检验值发生变化,影响相关疾病的诊断准确。因此，实验室的新收的不能及时检测的生化标本必须尽快地分离血清，放于4℃冰箱内或者冷冻起来，以免影响检测的结果，从而对临床疾病的诊疗造成影响。“,”Objective: To investigate the effect of laying aside time and treatment method on multiple biochemical results of blood samples. Methods: Three replicate blood samples were taken from 101 patients. The first tube were centrifuged and separated the serum immediately to determine the biochemical indexes; the second tube were centrifuged and stored at 4℃, examination were taken after a 24-hour-storage; the third tube stored at 4℃without centrifugation and determination were also taken after 24 hours. 36 biochemical indexes, i.e. ALT, AST, ALP, GGT, TP, ALB, AFU, CK, were selected to be determined according to the subjects our laboratory carrying out. Results: Compared with the biochemical indexes of immediate blood samples, AST, LDH, HBDH, CKMB and Zn increased after laying aside for 24 hours, and ALP, GGT, TP, ALB, AFU, T-BIL, D-BIL, CRE, UA, Glu, Cyc-sC, TG, HDL-C, ApoA, RF, CRP and AMS decreased. It has statistical significance (P<0.05). Compared with the samples of immediate centrifugation, AST, BUN, HBDH, CKMB, LDH, HBDH, CK, CKMB, Mg and Zn increased, and ALP, GGT, ALB, T-BIL, D-BIL, CRE, Glu, CHO, ApoA, ApoB, RF, CA and AMS decreased of the samples not being centrifuged (P<0.05). The examination of serums after storage of 24 hours showed, AST, TP, BUN, Cys-C, LDH, HBDH, CRP, Zn increased and ALP, T-BIL, D-BIL, CRE, Glu , ApoB, ASO, CA decreased of serum separated immediately compared with the serum separated after 24 hours (P<0.05).Conclusions: The examination proved that the biochemical data would altered on account of different laying aside time and treatment methods and influence the diagnosis of relative diseases. Therefore, serum should be separated and stored at 4℃as soon as possible after the blood specimens were received, so as to avoid the distortion of the results and subsequent misdiagnosis.