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为探究利用施氏假单胞菌代谢产物——氮气加固可液化砂土的可行性,对该菌反硝化作用的条件与砂样中产气效能进行了研究,分析了不同碳源对NO_3~--N与NO-2-N还原率的影响以及温度和初始p H值对其最终饱和度、平均产气速率、初始停滞期的影响,并确定了最优碳源、适用温度和初始p H值区间.试验结果表明,柠檬酸钠为最优碳源,该菌在21 h时对NO_3~--N还原率达到100%,NO-2-N存在先累积后还原的过程,并伴有0.82 mmol/L残留浓度.该菌在4~30℃下均可产气.随温度升高,平均产气速率大幅增加,砂样最终饱和度略有降低.恒温20℃且初始p H值为5~9时,该菌可在砂样中顺利产气.中性及碱性环境下砂样的最终饱和度基本一致,酸性环境下砂样的最终饱和度随初始p H值的减小而降低,细菌在砂样中的平均产气速率随初始p H值的下降而减小.与已有微生物气法相比,将该菌应用于IPS技术中具有平均产气速率快、初始停滞期短、工艺简单等优点.
In order to explore the feasibility of using nitrogen metabolites - nitrogen liquefaction of liquefiable sand, the conditions of denitrification and the gas production efficiency of sand samples were studied. The effects of different carbon sources on NO_3 ~ -N and NO-2-N reduction rate and the effects of temperature and initial p H on its final saturation, average gas production rate and initial stagnant period, and the optimal carbon source, applicable temperature and initial p H Value interval.The results showed that sodium citrate was the optimal carbon source, the rate of reduction of NO_3 ~ --N reached 100% at 21 h, NO-2-N was first accumulated and then reduced, accompanied by 0.82 mmol / L residual concentration.The bacteria could produce gas at 4 ~ 30 ℃ .The average gas production rate increased with the increase of temperature, the final saturation of sand sample slightly decreased.The initial p H value was The bacteria could produce gas smoothly in the sand sample from 5 to 9, and the final saturation of the sand sample was basically the same in the neutral and alkaline environments. The final saturation of the sand sample in acidic environment decreased with the initial p H value decreasing Decreased and the average gas production rate of bacteria in the sand sample decreased with the decrease of the initial p H value. Compared with the existing microbial gas method, the bacteria were used in the IPS technology An average gas production rate, short initial lag phase, simple process, etc..