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建立昆明小鼠受孕模型,分离并体外培养胚胎细胞.检测了各培养浓度下的细胞增殖、分化与凋亡.胚胎细胞在0.2mmol/L和5.56mmol/L葡萄糖浓度的KSOM培养基中能正常发育和孵化;而在浓度为15.56mmol/L和25.56mmol/L葡萄糖培养基中胚胎发育和孵化均受到损害(P<0.005),且总细胞数和内细胞团细胞数也明显减少(P<0.01),但其细胞凋亡率与0.2mmol/L和5.56mmol/L葡萄糖浓度下胚胎细胞凋亡率无显著性差异(P>0.05).随着葡萄糖浓度的增高,胚泡总的表面积无明显变化,但胚胎细胞密度呈增加趋势.高血糖对早期胚胎的发育具有毒性作用,提示高糖可能导致妊娠合并糖尿病患者的流产和胎儿畸形率升高.
Kunming mouse model of pregnancy was established, and the embryonic cells were isolated and cultured in vitro.Propagation, differentiation and apoptosis were detected at various culture concentrations.The embryonic cells were normal in KSOM medium with 0.2mmol / L and 5.56mmol / L glucose concentration (P <0.005), and the total cell number and the number of inner cell mass cells were significantly decreased (P <0.05). However, embryonic development and hatching were impaired at concentrations of 15.56 mmol / L and 25.56 mmol / L glucose medium 0.01), but the rate of apoptosis was not significantly different with the apoptosis rate of embryos at 0.2mmol / L and 5.56mmol / L glucose (P> 0.05) .With the increase of glucose concentration, the total surface area of blastocyst But the density of embryo cells tended to increase.Hyperglycemia has a toxic effect on the development of early embryos, suggesting that high glucose may cause miscarriage and fetal malformation in patients with gestational diabetes mellitus.