论文部分内容阅读
用抗促黄体激素(LH)单克隆抗体为试样,进行了叠氮钠(NaN_3)在ELISA检测中影响辣根过氧化物酶(HRP)活性的研究。实验结果表明,当酶标二抗工作液中不舍NaN_3时,含0.02％NaN_3的ODP底物工作液能将其中的HRP全部失活;而当底物工作液中不舍NaN_3时,含0.02％NaN_3的酶标二抗工作液中的HRP有30％～60％失活。提出在以HRP作为标记酶的ELISA检测所用的部分缓冲液中废止使用NaN_3作为防腐剂。
The anti-luteinizing hormone (LH) monoclonal antibody was used as a sample to study the effect of sodium azide (NaN_3) on the activity of horseradish peroxidase (HRP) in ELISA. The experimental results show that, when the enzyme-labeled secondary antibody working solution is not NaN_3, the working solution of ODP substrate containing 0.02% NaN_3 can inactivate all of HRP, while when the substrate working solution is not NaN_3-containing 0.02 HRP in% NaN_3 ELISA secondary antibody working solution was 30% ~ 60% inactivated. It is proposed to abolish the use of NaN_3 as a preservative in a portion of the buffer used for the ELISA assay using HRP as a labeling enzyme.