论文部分内容阅读
目的:观察研究近红外荧光染料IR-783在膀胱癌中的特异性成像。方法:通过染料与膀胱癌细胞及正常细胞共孵育,观察近红外荧光染料IR-783是否能够实现膀胱癌细胞的选择性成像。利用细胞器示踪剂观察近红外荧光染料在膀胱癌细胞内的共定位;使用IR-783检测循环血液中及尿液中的膀胱癌细胞。结果:近红外荧光染料IR-783可被膀胱癌细胞选择性摄取。IR-783可选择性聚集在膜性细胞器如线粒体和溶酶体内,这种选择性聚集作用使IR-783可以保持较长的染色效果。近红外染料可以检测到血液或尿液极少量的膀胱癌细胞。结论:近红外荧光染料IR-783能够被膀胱癌细胞特异性吸收,可用于血液和尿液中膀胱肿瘤细胞的特异性诊断,具有重要的临床应用前景。
Objective: To observe the specific imaging of near infrared fluorescent dye IR-783 in bladder cancer. Methods: The cells were incubated with human bladder cancer cells and normal cells to observe whether IR-783 can selectively display bladder cancer cells. The use of organelle tracer to observe the near-infrared fluorescent dye colocalization in bladder cancer cells; the use of IR-783 detection of circulating blood and urine of bladder cancer cells. Results: Near infrared fluorescence dye IR-783 can be selectively taken up by bladder cancer cells. IR-783 selectively accumulates in membranous organelles such as mitochondria and lysosomes. This selective aggregation allows IR-783 to maintain a long-lasting staining effect. Near infrared dyes can detect very little blood or urine bladder cancer cells. Conclusion: IR-783 can be specifically absorbed by bladder cancer cells and can be used for the specific diagnosis of bladder tumor cells in blood and urine. It has important clinical application prospects.