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探讨两种前处理方法在测定一次性纸杯水提取物的细胞毒性中的应用。一种前处理方法采用欧盟关于纸和纸板水提取物细胞毒性测定标准中的前处理方法,另一种前处理方法则模拟了纸杯日常使用条件。选用4种市售的一次性纸杯,对两种方法得到的纸杯浸提液使用MTT法测定了对CHO细胞的毒性作用。结果表明,4种受试纸杯使用本研究所采用两种方法的水提取物均未显示出明显细胞毒性,细胞相对增殖率无显著差异,两种前处理方法均可用于一次性纸杯的细胞毒性测定。
To investigate the application of two pretreatment methods in the determination of cytotoxicity of disposable paper cup water extract. One of the pre-treatment methods uses the EU pre-treatment method for the determination of cytotoxicity of paper and cardboard water extracts and the other pre-treatment method simulates the daily use conditions of paper cups. Four commercially available disposable paper cups were used to determine the toxicity of the two methods on the toxicity of CHO cells using the MTT method. The results showed that the water extracts of the four kinds of paper cups used in this study did not show any significant cytotoxicity, no significant difference in relative cell proliferation rate, the two pretreatment methods can be used for disposable cups of cytotoxicity Determination.