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目的:小白菊内酯(Parthenolide)可通过不同的途径影响肿瘤的进展,但有关其对结肠癌细胞的生物学行为的影响报道较少。本研究的目的就是探讨Parthenolide干预在结肠癌细胞生长、迁移和凋亡中的生物学作用,以及其对结肠癌细胞分泌VEGF-C的影响。应用QRT-PCR、Wstern Blot、免疫组织化学、细胞增殖、迁移及凋亡实验检测Parthenolide干预后结肠癌细胞生物学行为的变化。结果:parthenolide干预后48h开始抑制细胞活性(P<0.05);单独应用parthenolide不能影响结肠癌细胞的迁移和凋亡;parthenolide处理后可抑制结肠癌细胞分泌VEGF-C。尾静脉注射Parthenolide可抑制移植瘤生长、淋巴管生成。结论:这些研究表明parthenolide能有效抑制结肠癌细胞及移植瘤的生长,其可能成为潜在的抗癌药物。
OBJECTIVE: Parthenolide can affect tumor progression through different pathways, but few reports about its effect on the biological behavior of colon cancer cells. The purpose of this study was to investigate the biological role of Parthenolide in the growth, migration and apoptosis of colon cancer cells and its effect on the secretion of VEGF-C by colon cancer cells. The changes of biological behaviors of colon cancer cells after Parthenolide intervention were detected by QRT-PCR, Wstern Blot, immunohistochemistry, cell proliferation, migration and apoptosis assay. RESULTS: Parthenolide inhibited the cell viability at 48h (P <0.05). Parthenolide alone did not affect the migration and apoptosis of colon cancer cells. Parthenolide inhibited the secretion of VEGF-C by colon cancer cells. Parthenolide can inhibit xenograft tumor growth and lymphangiogenesis by tail vein injection. Conclusion: These studies indicate that parthenolide can effectively inhibit the growth of colon cancer cells and xenografts and may be a potential anti-cancer drug.