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1植物名称闽赣长蒴苣苔(Didymocarpus heucherifoliusHand.-Mazz.)。2材料类别幼叶。3培养条件MS为基本培养基。诱导不定芽分化培养基:(1)MS+6-BA0.1 mg·L~(-1)(单位下同)+NAA0.1;(2)MS+6-BA 1.0+NAA 0.5;(3)MS+6-BA 2.0+NAA0.5。增殖继代培养基:(2);(3);(4)MS+6-BA 2.0+NAA 1.0;(5)MS+6-BA 0.05+NAA 0.05。生根培养基:(6)1/2MS+NAA 0.5;(7)1/2MS+0.5%活性炭;(8)1/2MS+0.1%活性炭。以上培养基均含30 g·L~(-1)蔗糖和7.0 g·L~(-1)琼脂,pH 5.8。培养温度为(25±2)℃,光照强度约为30μmol·m~(-2)·s~(-1),光照时间为16h.d~(-1)。
1 plant name Fujian and Jiangxi Ganoderma long potted lichen (Didymocarpus heucherifoliusHand.-Mazz.). 2 material categories young leaves. 3 culture conditions MS as the basic medium. Induction of adventitious bud differentiation medium: (1) MS + 6-BA0.1 mg · L -1 (unit same below) + NAA0.1; (2) MS + 6- BA 1.0 + NAA 0.5; ) MS + 6-BA 2.0 + NAA 0.5. Proliferation subculture medium: (2); (3); (4) MS + 6-BA 2.0 + NAA 1.0; (5) MS + 6-BA 0.05 + NAA 0.05. Rooting medium: (6) 1/2 MS + NAA 0.5; (7) 1/2 MS + 0.5% activated carbon; and (8) 1/2 MS + 0.1% activated carbon. The above medium contained 30 g · L -1 sucrose and 7.0 g · L -1 agar at pH 5.8. The incubation temperature was (25 ± 2) ℃, the light intensity was about 30μmol · m -2 · s -1 and the light time was 16h.d -1.