目的：进一步研究茶多酚（ＴＰ）在体外细胞毒作用、体内抗肿瘤的作用。方法：噻唑兰（ＭＴＴ）体外试验法和灌胃给药体内抗瘤试验。结果：茶多酚对体外培养的人鼻咽癌细胞ＣＮＥ２、人肺腺癌Ａ５４９细胞和ＧＬＣ８２细胞及乳腺癌细胞ＭＣＦ７的半数抑制浓度（ＩＣ５０）依次为１０２７２μｇ／ｍｌ、３５７６μｇ／ｍｌ、７００μｇ／ｍｌ和６３１０μｇ／ｍｌ。体内灌胃给予ＴＰ１２５ｍｇ／ｋｇ、５０ｍｇ／ｋｇ和２０ｍｇ／ｋｇ·ｄ×１０天剂量下对小鼠肿瘤Ｌ２的抑瘤率依次为３１０６～３０９９％、４２０５～４９７５％和４５１２～４６７９％，Ｐ＜００５～００１。ＴＰ在１２５ｍｇ／ｋｇ、５０ｍｇ／ｋｇ和２０ｍｇ／ｋｇ·ｄ×１８天剂量下，对人肺癌裸鼠移植瘤的抑瘤率依次为２９７７％、３７４１％和４８３４％，后二者Ｐ＜００５～００１，有显著性意义。结论：茶多酚在体外显示细胞毒作用，体内试验有抗肿瘤作用 Objective: To further study the role of tea polyphenols (TP) in vitro cytotoxicity and in vivo anti-tumor effect. Methods: Thiazide blue (MTT) in vitro test and oral administration of anti-tumor test in vivo. Results: The median inhibitory concentration (IC50) of tea polyphenols on human nasopharyngeal carcinoma cells CNE2, human lung adenocarcinoma A549 cells, GLC82 cells and breast cancer cells MCF7 were 10272 μg / ml, 35 76μg / ml, 700μg / ml and 6310μg / ml. The antitumor rates of mice treated with TP1.25mg / kg, 50mg / kg and 20mg / kg · d × 10days were 3106 ~ 3099% and 4205 ~ 4975% and 4512 ​​~ 4679%, P <005 ~ 001. The inhibitory rate of TP to xenografts in nude mice bearing human lung cancer were 2977%, 3741% and 48%, respectively, at 125mg / kg, 50mg / kg and 20mg / kg · d × 18days 34%, the latter two P <0  05 ~ 0  01, a significant significance. Conclusion: Tea polyphenols show cytotoxicity in vitro and anti-tumor effect in vivo