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目的比较研究多重荧光逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)和传统RT-PCR方法对手足口病(Hand,Foot and Mouth Disease,HFMD)检测结果的影响。方法采集HFMD患儿轻症门诊病例咽拭子标本86份、重症住院病例咽拭子120份,采用多重荧光和传统RT-PCR方法进行检测并比较分析。结果多重荧光RT-PCR较传统RT-PCR对咽拭子标本中肠道病毒(Enterovirus,EV)的检出率有显著提高。轻症病例的检出率由52.3%提高至93.0%,重症病例的检出率由8.3%提高至60.0%。EV 71型、柯萨奇病毒A组16型及其他EV在HFMD病原中的构成比也相应发生了改变。结论在对HFMD咽拭子标本的检测中,尤其是对于重症病例,多重荧光RT-PCR较传统RT-PCR更灵敏,能够更真实地反映HFMD疫情的病原构成。
Objective To compare the effects of multiple fluorescence reverse transcription-polymerase chain reaction (RT-PCR) and traditional RT-PCR on the detection of Hand, Foot and Mouth Disease (HFMD). Methods Totally 86 throat swabs and 120 throat swabs from 120 intensive-care outpatients with HFMD were collected and analyzed by multiplex fluorescence and conventional RT-PCR. Results Compared with traditional RT-PCR, multiplex fluorescence RT-PCR significantly increased the detection rate of enterovirus (EV) in throat swab specimens. The detection rate of mild cases increased from 52.3% to 93.0%, the detection rate of severe cases increased from 8.3% to 60.0%. The compositional ratio of EV 71, Coxsackievirus A, type 16 and other EVs in HFMD pathogens has also changed accordingly. Conclusion In detecting HFMD throat swab specimens, especially for severe cases, multiplex fluorescence RT-PCR is more sensitive than traditional RT-PCR and can more accurately reflect the etiopathogenisis of HFMD epidemic.