用诱导分化前、后的两株人白血病细胞系K562和HL-60细胞分别与正常骨髓细胞混合培养作为实验模型,研究白血病细胞对正常骨髓祖细胞生长的抑制。诱导剂处理前或后的10~4个K562或HL-60细胞与肋骨骨髓细胞(2.5—5×10~4个)做血浆凝块和/或血浆凝块-琼脂双层培养,于培养后第7和12天,用联苯胺-苏木素染色,分别计数CFU-E和BFU-E。K562和HL-60细胞对CFU-E、BFU-E的生长抑制率分别为:CFU-E40—60％、BFU-E54.8±2.3％。随着白血病细胞数的增加对正常骨髓细胞的抑制率也升高,当加至10~6个K562细胞与正常骨髓混合培养,则无BFU-E生长。这种抑制作用也存在于K562细胞的条件培养液中。用30—70μmol氯高铁血红素(或2μmol丁酸钠)和2μmol顺-视黄酸处理K562和HL-60细胞,在第2,3、4、5天分别收集之,用上述方法测定它们对CFU-E、BFU-E集落形成的影响。HL-60细胞用视黄酸处理4天后就失去对BFU-E的抑制作用,K562细胞用丁酸钠诱导后有同样的结果。并且,随着处理时间 Two human leukemia cell lines K562 and HL-60 before and after differentiation were mixed with normal bone marrow cells as an experimental model to study the inhibition of growth of normal myeloid progenitor cells by leukemic cells. Ten to four K562 or HL-60 cells and rib bone marrow cells (2.5-5×10-4) before or after inducer treatment were used for plasma clot and/or plasma clot-agar bilayer culture after culture. On days 7 and 12, they were stained with benzidine-hematoxylin and counted for CFU-E and BFU-E, respectively. The inhibition rates of CFU-E and BFU-E in K562 and HL-60 cells were: CFU-E 40-60% and BFU-E 54.8±2.3%. With the increase of the number of leukemia cells, the inhibition rate of normal bone marrow cells also increased. When 10 to 6 K562 cells were mixed with normal bone marrow, there was no BFU-E growth. This inhibition is also present in the conditioned medium of K562 cells. K562 and HL-60 cells were treated with 30-70 μmol of hemin (or 2 μmol of sodium butyrate) and 2 μmol of cis-retinoic acid and collected on days 2, 3, 4, and 5, respectively. Effects of CFU-E and BFU-E colony formation. HL-60 cells lost their inhibitory effect on BFU-E 4 days after treatment with retinoic acid, and K562 cells had the same results when induced with sodium butyrate. And, with the processing time