论文部分内容阅读
目的:通过观察老年抑郁大鼠模型的心血管活性物质一氧化氮、内皮素-1(NO、ET-1)变化,了解抑郁与心血管疾病的相关性;同时观察调脂积冲剂的可能干预作用。方法:Wistar老年大鼠共32只,随机分为正常空白组、模型对照组、调脂积冲剂干预组、氟西汀干预组。适应性喂养后,给予持续性温和刺激(包括冰水游泳、热刺激、禁食、禁水、夹尾、昼夜颠倒、高速水平摇晃、频闪灯照射)造抑郁模型。经检测造模成功后观察大鼠体质量变化、糖水偏嗜等一般情况,同时检测血管活性物质(NO、ET-1)水平。结果:给予持续性温和刺激56 d后,模型组与正常组比较,糖水偏嗜度有降低趋势,但无明显差异(P>0.05);调脂积冲剂组、氟西汀组与模型组比较,糖水偏嗜度有明显升高,其中调脂积冲剂组差异明显(P<0.05)。模型组与正常组比较,NO水平明显升高(P<0.05);调脂积冲剂组、氟西汀组与模型组比较,NO水平有下降趋势,其中调脂积冲剂组差异显著(P<0.05)。模型组与正常组比较,ET-1水平明显升高(P<0.05);调脂积冲剂组、氟西汀组与模型组比较,ET-1水平均呈下降趋势,氟西汀组差异明显(P<0.05)。结论:老年抑郁大鼠模型相关心血管活性物质(NO、ET-1)可产生变化,可能以此途径影响心血管疾病的发病及预后;调脂积冲剂对老年抑郁大鼠模型的行为及相关心血管活性物质具有干预作用。
OBJECTIVE: To investigate the relationship between depression and cardiovascular diseases by observing the changes of the cardiovascular active substances nitric oxide and endothelin-1 (NO, ET-1) in senile depression rat model and to observe the possible intervention effect. Methods: A total of 32 old Wistar rats were randomly divided into normal control group, model control group, intervention group with lipid-lowering agent and fluoxetine intervention group. After adaptive feeding, depression was modeled by sustained mild stimulation (including ice-water swimming, thermal stimulation, fasting, water forbidding, pinching, day and night reversal, shaking at high speed and stroboscopic light irradiation). After the success of the test, the body mass of the rats was observed, the general situation of the carnitine and other side effects were observed, and the levels of vasoactive substances (NO, ET-1) were also detected. Results: After 56 days of sustained moderate stimulation, compared with the normal group, the degree of glycemic deviations tended to decrease, but there was no significant difference (P> 0.05). Compared with model group, Tiazhijia granules group and fluoxetine group (P <0.05). There was a significant difference in the degree of partial acidity of glycogen between the two groups (P <0.05). Compared with the model group, the level of NO in the model group was significantly increased (P <0.05). Compared with the model group, the NO level decreased in Tiazhijia granules group and fluoxetine group, and there was significant difference among them (P < 0.05). The levels of ET-1 in the model group were significantly higher than those in the normal group (P <0.05). Compared with the model group, the levels of ET-1 in Tiazhijili Granule group and fluoxetine group showed a decreasing trend, and the fluoxetine group was significantly different (P <0.05). CONCLUSIONS: The cardiovascular related substances (NO, ET-1) in the senile depression rat model may be changed, which may affect the pathogenesis and prognosis of cardiovascular diseases in this way. The behavior and relatedness of Tiaozhi Capsule in senile depression rat model Cardiovascular active substances have an interfering effect.