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目的比较脂多糖(LPS)和盐酸(HCl)两种诱因致急性肺损伤(ALI)的差异。方法 30只健康雄性SD大鼠随机均分为三组:生理盐水(NS)组(尾静脉注射)、LPS组(尾静脉注射)、HCl组(气管内滴入)。制模后观察4h处死,检测动脉血气及支气管肺泡灌洗液(BALF)中细胞总数、中性粒细胞(PMN)百分比、蛋白含量和肺湿/干重比(W/D)、血清和BALF中肿瘤坏死因子α(TNF-α),观察肺组织病理形态学改变并进行病理损伤评分。结果 LPS组、HCl组氧分压(PaO2)均低于NS组(P<0.01),且HCl组较LPS组更低(P<0.01);两组肺W/D、BALF中细胞总数、PMN%、蛋白含量、血清和BALF中TNF-α水平、ALI病理评分均明显高于NS组(P<0.01)。除肺W/D和ALI病理评分外,BALF中细胞总数、PMN%、蛋白含量、TNF-α水平HCl组均明显高于LPS组(P<0.01)。结论 ALI的发生发展与其致病因素密切相关;不同病因所致的ALI存在一定差异。
Objective To compare the differences of acute lung injury (ALI) induced by two kinds of lipopolysaccharide (LPS) and hydrochloric acid (HCl). Methods Thirty healthy male Sprague-Dawley rats were randomly divided into three groups: saline (NS) group (tail vein), LPS group (tail vein) and HCl group (intratracheal instillation). The rats were sacrificed at 4h after model establishment. The total number of cells, percentage of neutrophils (PMN), protein content and W / D (wet / dry weight ratio), serum and BALF were measured in arterial blood gas and bronchoalveolar lavage fluid In tumor necrosis factor alpha (TNF-α), observe the pathological changes of lung tissue and pathological damage score. Results The oxygen partial pressure (PaO2) in LPS group and HCl group was lower than that in NS group (P <0.01), and the HCl group was lower than LPS group (P <0.01). The total cell number, PMN %, Protein content, TNF-α level in serum and BALF and ALI pathological score were significantly higher than those in NS group (P <0.01). In addition to lung W / D and ALI pathological score, the total number of cells in BALF, PMN%, protein content, TNF-α levels were significantly higher in the HCl group than in the LPS group (P <0.01). Conclusions The occurrence and development of ALI are closely related to the pathogenesis of ALI. There are some differences in ALI caused by different etiologies.