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目的 :建立测定人血浆中阿替洛尔浓度的HPLC法。方法 :采用混合有机试剂 (氯仿∶异丙醇 ,75∶25,V/V)萃取样品中的药物 ,对干燥后的残渣用流动相复溶 ,应用高效液相色谱法分离 ,采用荧光检测器检测 (检测波长Ex=225nm ,Em=300nm)。流动相为乙腈∶水∶1mol/L磷酸缓冲液6∶89∶5(V/V/V) ,pH=4 ,流速1ml/min ,内标为美托洛尔。结果 :血浆中阿替洛尔在15.6～1000.0ng/ml范围内 ,药物浓度与峰面积比值呈良好的线性关系 (r=0.9996 ,RUN=3)。总回收率为98.78 %±7.96 %。日内和日间差异均小于10%。最低检出限为10ng/ml。结论 :用改进后的高效液相色谱法测定人血浆中阿替洛尔的浓度具有对样品纯化程度高、分析周期短等优点 ,实际应用效果良好
Objective: To establish an HPLC method for the determination of atenolol in human plasma. Methods: The samples were extracted with mixed organic reagent (chloroform: isopropanol, 75:25, V / V). The dried residue was reconstituted with mobile phase and separated by high performance liquid chromatography. The fluorescence detector Detection (detection wavelength Ex = 225nm, Em = 300nm). The mobile phase consisted of acetonitrile: water: 1 mol / L phosphate buffer 6:89:5 (V / V / V), pH = 4 and flow rate 1 ml / min. The internal standard was metoprolol. Results: The plasma concentration of atenolol was in the range of 15.6-1000.0 ng / ml. There was a good linear relationship between drug concentration and peak area ratio (r = 0.9996, RUN = 3). The total recovery was 98.78% ± 7.96%. Differences between days and days are less than 10%. The minimum detection limit is 10ng / ml. Conclusion: The determination of atenolol in human plasma with improved HPLC has the advantages of high purification, short analysis period, and good practical application