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本文运用聚合酶链式反应技术(PCR)对新疆和田地区30例90岁以上维吾尔族长寿老人和70例当地健康、无血缘关系、生活习惯相似、随机抽样的维吾尔族对照人群进行5-HTTLPR基因分型及相应等位基因频率的比较并且探讨了5-羟色胺转运体基因连锁多态性区域(5-HTTLPR)的44bp插入/缺失序列多态性与长寿的相关性。结果表明,维吾尔族长寿老人的5-HTTLPR基因多态性的LL(528bp/528bp)基因型和L(528bp)等位基因频率分别为70%和81.7%,对照者LL基因型和L等位基因频率分别为40%和57.2%,两组之间比较差异有显著性(P<0.05)。由此得出,5-HTTLPR基因多态性与新疆和田地区维吾尔族老人的长寿有关联,新疆和田地区长寿相关基因组成为LL基因型和L等位基因。
In this study, polymerase chain reaction (PCR) was used to analyze the prevalence of 5-HTTLPR gene in 30 Uighur long-term elderly over 90 years old in Hetian area and 70 healthy Unear-controlled, randomized, Type and the corresponding allele frequency and to explore the serotonin transporter gene-linked polymorphic region (5-HTTLPR) 44bp insertion / deletion sequence polymorphism and longevity correlation. The results showed that the frequencies of LL (528bp / 528bp) and L (528bp) alleles of 5-HTTLPR gene polymorphism in Uygur longevity elderly were 70% and 81.7% respectively. The LL genotype and L allele The frequencies were 40% and 57.2%, respectively, with significant difference between the two groups (P <0.05). It was concluded that the 5-HTTLPR gene polymorphism was associated with the longevity of Uygur elders in Hetian, Xinjiang, and the longevity-related genes in Hetian were LL genotype and L allele.