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以新疆主栽核桃品种温185、新2、露仁、扎343的1年生枝条为材料,通过低温梯度(-15、-18、-21、-24、-27、-30、-33、-36℃)处理12h,测定各品种枝条电解质外渗率、可溶性糖、可溶性蛋白、脯氨酸含量等生理指标,利用隶属函数法对4个新疆主栽核桃品种抗寒性进行了综合评价。结果表明,随温度的降低,测定指标都出现S形变化趋势。其中,扎343、露仁枝条的电解质外渗率和可溶性糖含量-30℃时达到峰值,然后降低,可溶性蛋白含量峰值在-27℃时出现,脯氨酸含量峰值出现较早,扎343枝条的脯氨酸含量在-18℃时达到峰值,露仁枝条的脯氨酸含量在-21℃时达到峰值;新2枝条的相对电解质外渗率和脯氨酸含量在-30℃时达到峰值,然后降低,可溶性糖含量在-21℃时达到峰值,可溶性蛋白质含量在-27℃时达到峰值;温185的电解质外渗率和可溶性糖含量在-36℃时达到峰值,可溶性蛋白质含量在-24℃时达到峰值,脯氨酸含量在-18℃时达到峰值。根据综合评判结果,核桃品种温185、新2、露仁、扎343的平均隶属度为0.413~0.497,4个核桃品种的抗寒性排序为:新2>温185>露仁>扎343。
The annual branches of walnut varieties Weng 185, Xin 2, Lu Ren and Zha 343 planted in Xinjiang were used to study the effects of low temperature gradient (-15, -18, -21, -24, -27, -30, -33, - 36 ℃) for 12 h. The physiological indexes such as electrolyte leakage rate, soluble sugar, soluble protein and proline content were measured. The cold resistance of four main cultivars of walnut in Xinjiang were comprehensively evaluated by membership function. The results show that with the decrease of temperature, the S-shaped trend of the measured indicators appears. Among them, Zha 343, the electrolyte leakage rate and the content of soluble sugar of almond branches peaked at -30 ℃, then decreased, the peak of soluble protein content appeared at -27 ℃, the peak of proline content appeared earlier and 343 branches Proline content peaked at -18 ℃, and proline content peaked at -21 ℃. The relative electrolyte leakage rate and proline content of the new two branches peaked at -30 ℃ , And then decreased. The content of soluble sugar peaked at -21 ℃, the content of soluble protein peaked at -27 ℃. The electrolyte leakage rate and soluble sugar content reached its peak at -36 ℃ in temperature 185 and the content of soluble protein reached - The peak was reached at 24 ° C and the proline content peaked at -18 ° C. According to the results of comprehensive evaluation, the average membership degree of walnut variety 185, Xin 2, Lu Ren and Zha 343 was 0.413 ~ 0.497, and the order of cold resistance of 4 walnut cultivars was Xin 2> Wen 185> Lu Ren> Zha 343.