论文部分内容阅读
以１９９０～１９９３年从河南省不同地区采集的３６４个小麦白粉病菌菌株标样为例，应用传统的生理小种鉴定方法和根据与已知抗白粉基因的互作推测毒性基因频率的方法，研究了河南省小麦白粉病菌群体生理小种和毒性基因结构。结果发现白粉病菌群体中生理小种组成及其频率有较大变化，亚号、１０号、１１号和１５号小种的频率均呈下降趋势，而３１号小种的频率呈大幅度上升，至１９９３年已达２０．７５％，跃居各小种之首；对１号、１１号、１５号和３１号小种的毒性基因谱分析发现，不同小种群的小种间至少存在一个以上毒性基因的差异，同小种群的不同小种间则在毒性基因谱上没有显著差异；另外，同一小种不同菌株间的毒性基因谱仍不完全相同，说明小种致病异质性是普遍存在的。毒性基因结构分析表明，Ｖ２、Ｖ２＋６和Ｖ８等毒性基因的频率有较大变化。文中还讨论了两种方法的优缺点和今后工作中如何改进等问题。
Taking 364 strains of powdery mildew of wheat powdery mildew collected from different areas in Henan Province from 1990 to 1993 as an example, the traditional method of identifying race of races and the method of estimating the frequency of virulence genes according to the interaction with known anti-white powder genes were studied Physiological races and virulence genes of powdery mildew of wheat in Henan Province were studied. The results showed that the composition and frequency of races of powdery mildew were changed greatly. The frequencies of subspecies number 10, number 11 and number 15 showed a decreasing trend, but the frequency of number 31 raises significantly, Reaching 20.75% in 1993, jumping to the first of the races. Toxic gene spectrum analysis of races 1, 11, 15 and 31 found that there were at least one more races in different races The difference of virulence genes between different races of the same small population showed no significant differences in the virulence gene spectrum. In addition, the virulence gene profiles of different races of the same races were still not identical, which indicated that the racial heterogeneity of races was common existing. Toxicity gene structure analysis showed that V2, V2 + 6 and V8 and other toxic genes have greater changes in the frequency. The article also discusses the advantages and disadvantages of the two methods and how to improve the work in the future.