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目的:对山羊角提取物中的焦谷氨酸进行含量测定研究。方法:通过氨基酸自动分析仪及傅立叶变换离子回旋共振质谱确认山羊角提取物中含有焦谷氨酸,并建立专属高效液相色谱方法对焦谷氨酸进行含量检测。色谱条件为:色谱柱为Sion-chrom ODS-BP(200 mm×4.6 mm,5μm),流动相为乙腈-3.5 mmo.lL-1磷酸氢二钠缓冲液(1∶99),调pH=3,流速为1.0mL.m in-1,检测波长205 nm,柱温27℃。结果:高分辨质谱有m/z128离子峰,并且检测出该批山羊角提取物中含有焦谷氨酸7.65%。结论:所建立的方法可快速、稳定地检测山羊角提取物中的焦谷氨酸,从而有效提高山羊角提取物的检测标准。
Objective: To study the determination of pyroglutamic acid in goat horn extract. Methods: The contents of pyroglutamic acid in goat horn extract were confirmed by automatic amino acid analyzer and Fourier transform ion cyclotron resonance mass spectrometry (HPLC-MS / MS). The chromatographic conditions were as follows: the column was Sion-chrom ODS-BP (200 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-3.5 mmo.lL-1 dibasic sodium phosphate buffer , The flow rate of 1.0mL.min-1, detection wavelength 205 nm, column temperature 27 ℃. Results: The m / z 128 ion peak was detected by high resolution mass spectrometry, and the pyglutamic acid 7.65% was detected in the goat horn extract. Conclusion: The established method can detect pyroglutamic acid in goat horn extract quickly and stably, so as to effectively improve the detection standard of goat horn extract.