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目的观察转苏云金杆菌CrylVD基因蓝藻实验室对淡色库蚊、白纹伊蚊和中华按蚊不同龄期幼虫杀灭效果。方法利用不同浓度的工程藻,分别作用于淡色库蚊、白纹伊蚊、中华按蚊敏感品系III龄幼虫,以及它们不同龄期的幼虫,并设计阴性及空白对照,分别于24H、48H计数死亡率,从而分析基因蓝藻的杀蚊幼毒效。结果工程藻对三种蚊种III龄幼虫均具有杀灭作用,对淡色库蚊、白纹伊蚊幼虫杀灭效果较高,对中华按蚊幼虫效果较差。48H对以上三种蚊虫III龄幼虫的杀灭率分别为100%、100%和42%;工程藻对低龄幼虫的杀灭效果高于高龄幼虫,即龄期越低对工程藻的敏感性越高,杀蚊毒效与龄期呈负相关。结论转苏云金杆菌CrylVD基因蓝藻具有明显杀蚊幼效果,为今后蚊虫防治提供了新的方法。
Objective To observe the killing effect of cyanobacterium CrylVD gene in cyanobacteria laboratory on larvae of Culex pipiens pallens, Aedes albopictus and Anopheles sinensis at different instars. Methods Different concentrations of engineering algae were applied to the third instar larvae of susceptible strains of Culex pipiens pallens, Aedes albopictus and Anopheles sinensis respectively, and their larvae at different instars were designed. Negative and blank controls were designed and counted respectively at 24H and 48H Mortality, to analyze the cyanobacteria mosquito nematocytosis effect. Results The engineered algae had a killing effect on all the three species of larvae of the third species of mosquitoes, killing larvae of Culex pipiens pallens and Aedes albopictus to a lesser extent. 48H killed 100%, 100% and 42% of the 3rd instar larvae of the above three mosquitoes respectively. The killing effect of engineering algae on younger larvae was higher than that of the older larvae, ie, the lower the age, the lower the sensitivity to engineering algae High, killing mosquito toxicity and age was negatively correlated. Conclusion Cyanobacteria carrying CrylVD gene of Bacillus thuringiensis have obvious mosquito-killing effect and provide a new method for mosquito control in the future.