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利用双向电泳及MALDI-TOF-MS技术分析绥农10号真叶接种疫霉菌1号生理小种后的蛋白质组变化。在抗病品种绥农10号叶片中共获得19个差异表达蛋白点,其中有12个上调表达,6个下调表达,1个特异表达(仅在接种后出现)。利用生物质谱分析8个上调表达点、1个下调表达点和1个特异表达点,最终鉴定得到8个有注释功能的蛋白,根据功能可分为4类,第1类为参与新陈代谢的蛋白,包括二磷酸核酮糖羧化酶的大亚基及前体、琥珀酰-辅酶A;第2类为参与信号传导的蛋白,包括激酶受体类蛋白、氧化还原酶和半胱氨酸氧化还原酶;第3类为参与细胞内物质运输的蛋白,包括衣壳蛋白的zeta-3亚基;第4类为转录因子,是参与茉莉酸介导的F-box蛋白。这些蛋白可为进一步研究大豆抗病机制奠定基础。
Proteomic analysis of Suinong 10 true leaf inoculated with Phytophthora race 1 was carried out by two dimensional electrophoresis and MALDI-TOF-MS. A total of 19 differentially expressed protein spots were obtained in the resistant variety Suinong 10 leaves, of which 12 were up-regulated, 6 down-regulated and 1 were specifically expressed (only after inoculation). Eight up-regulated expression spots, one down-regulated expression spot and one specific expression spot were analyzed by bio-mass spectrometry. Eight annotated proteins were finally identified and classified into four categories according to their functions. The first category was proteins involved in metabolism, Including the subunit and precursor of ribulose bisphosphate carboxylase, succinyl-CoA; class 2 is a protein involved in signal transduction including kinase receptor-like proteins, oxidoreductases and cysteine redox The third type is the zeta-3 subunit of the capsid protein involved in the transport of intracellular substances. The fourth type is the transcription factor involved in the jasmonic acid-mediated F-box protein. These proteins can lay the foundation for further research on the mechanism of disease resistance in soybean.