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目的了解安徽省阜阳市流行的狂犬病毒株与人用、兽用狂犬病疫苗株在G基因核苷酸和氨基酸水平的差异,为有效控制我国狂犬病疫情提供初步科学依据。方法在安徽省阜阳市收集犬脑组织162份,用酶联免疫吸附试验(ELISA)和小鼠颅内接种试验(MIT)检测样品带毒情况,对阳性样品用RT-PCR扩增G基因并测序,以TOPALi和DNA Star软件对G基因序列进行分析。结果从安徽省阜阳市162份犬脑样品中检出阳性样品15份;这15株病毒与我国现在使用的各种疫苗株在G基因的核苷酸和氨基酸水平上均存在不同程度的变异,与我国人用疫苗株CTN同源性较高。结论15株狂犬病毒为基因Ⅰ型狂犬病毒,在核苷酸或氨基酸水平上,与疫苗株CTN之间的同源性要高于与其它疫苗株之间的同源性。
Objective To understand the differences in nucleotide and amino acid levels of G gene between rabies virus strains and rabies virus strains for human and veterinary use in Fuyang City, Anhui Province, and to provide a preliminary scientific basis for the effective control of rabies epidemic in China. Methods 162 canine brain tissues were collected from Fuyang City, Anhui Province. The samples were infected with enzyme-linked immunosorbent assay (ELISA) and mouse intracranial inoculation test (MIT). The positive samples were amplified by RT-PCR Sequencing, the G gene sequence was analyzed with TOPALi and DNA Star software. Results A total of 15 positive samples were detected from 162 canine brain samples in Fuyang City, Anhui Province. The 15 strains of viruses showed different degrees of nucleotide and amino acid variation with the various vaccine strains currently used in China. And our human vaccine strains CTN homology is higher. Conclusion 15 strains of rabies virus are type Ⅰ rabies virus, which have higher homology with CTN of vaccine strain at the nucleotide or amino acid level than those of other vaccine strains.