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利用光学显微镜研究豌豆尖孢镰刀菌(Fusariumoxysporumf.sp.pisi)对豌豆根尖各部位的初侵染过程.结果表明:在不造成伤口的情况下,病菌在根尖的各部位均能直接成功侵入,但各部位侵入的频率不同,以伸长区侵入的频率最高.同时因各部位的木质化程度不同,侵染方式和结构也不同.当病菌从薄壁细胞侵入时,菌丝尖端形成锥状侵入丝直接侵入;当病菌从厚壁细胞侵入时,菌丝尖端形成垫状细胞附着于寄主细胞壁,后形成很细的侵入丝穿透细胞壁侵入.当菌丝侵入表皮细胞壁时,细胞壁内侧产生一个乳状突起阻止菌丝侵入.文中附示意图3幅,显微照片20张.
The initial infection of pea root tips with Fusarium oxysporum f.sp.pisi was studied by light microscopy. The results showed that the pathogen could directly invade in all parts of the root tip without causing wounds, but the frequency of invasion was different in different parts and the invasion in the elongation zone was the highest. At the same time due to the different parts of the degree of lignification, infection and structure are also different. When the bacteria invade from parenchyma cells, the conical invader filaments form at the tip of the hyphae to invade directly. When the bacteria invade from the thick-walled cells, the tip cells of the hyphae attach to the host cell wall and form a very thin invaded filament Cell wall invasion. When mycelium invades the epidermal cell wall, a milky protrusion is created inside the cell wall to prevent the mycelium from intruding. The text attached to the schematic 3, 20 photos.