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目的 探讨骨肉瘤伴神经内分泌分化的形态特征和组织发生。方法 应用光镜、电镜观察组织学结构和细胞形态 ;应用免疫组织化学 (ABC法 )检测 17例骨肉瘤中神经特异性烯醇化酶 (neuronspecificenolase ,NSE)、嗜铬素A(chromograniaA ,CgA)、突触素 (synaptophysin ,SY)和S 10 0蛋白的表达水平。结果 NSE、CgA、SY和S 10 0蛋白的阳性表达率分别为 82 % (14/ 17)、70 % (12 / 17)、5 2 % (9/ 17)和 88% (15 / 17) ,阳性细胞率分别为 2 5 %、2 0 %、2 1%和2 9%。阳性细胞的分布没有明显规律。 17例电镜观察 ,在部分瘤细胞内均见神经内分泌颗粒。结论 骨肉瘤伴神经内分泌分化起源于原始间叶细胞 ,并可分化为形态各异的瘤细胞。没有特殊的形态学特征
Objective To investigate the morphological and histological features of osteosarcoma with neuroendocrine differentiation. Methods The histological structure and cell morphology were observed by light microscopy and electron microscopy. The expression of neuron specific enolase (NSE), chromogranA (CgA) in 17 cases of osteosarcoma was detected by immunohistochemistry (ABC method) Synaptophysin (synaptophysin, SY) and S 10 0 protein expression levels. Results The positive rates of NSE, CgA, SY and S 10 0 protein were 82% (14/17), 70% (12/17), 52% (9/17) and 88% (15/17) The positive cell rates were 25%, 20%, 21% and 29%, respectively. No obvious regularity of the distribution of positive cells. 17 cases of electron microscopy, neuroendocrine granules were seen in some tumor cells. Conclusion Osteosarcoma with neuroendocrine differentiation originated in primitive mesenchymal cells and can differentiate into different morphological tumor cells. No special morphological features