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目的运用Plackett-Burman实验设计联用星点设计(central composite design,CCD)效应面法,考察各影响因素显著性,筛选出大黄地上部位(aerial part of Rheum officinale Baill,APOR)总蒽醌及大黄素提取的最佳工艺,为非药用部位的开发与应用提供依据。方法运用Plackett-Burman实验设计筛选主要影响因素,采用CCD-效应面积法优选APOR蒽醌提取工艺。以乙醇体积分数、提取时间、料液比为自变量,总蒽醌和大黄素含量为因变量,通过对自变量与因变量的完全二次响应曲面的回归拟合,利用三维曲面图直观分析APOR蒽醌提取最佳工艺,并进行预测分析。结果总蒽醌的最佳提取工艺为:乙醇体积分数67.25%,时间90 min,料液比28∶1;大黄素的最佳提取工艺为:乙醇体积分数85%,时间90 min,料液比40∶1。结论利用Plackett-Burman实验设计联用CCD确定了APOR总蒽醌和大黄素的提取工艺,该方法简便,精度更高、重现性好、预测性强。
OBJECTIVE To investigate the significance of various influencing factors by using Plackett-Burman experimental design combined with central composite design (CCD) surface methodology to screen anthraquinones and rhubarbs of aerial part of Rheum officinale Baill (APOR) Extraction of the best technology for non-medicinal parts of the development and application of the basis. Methods Plackett-Burman experimental design was used to screen the main influencing factors. The AP-anthraquinone extraction process was optimized by CCD-effect area method. The volume fraction of ethanol, extraction time, the ratio of solid to liquid as independent variable, total anthraquinone and emodin as dependent variables, by independent variables and dependent variable complete quadratic response surface fitting, using three-dimensional surface map intuitive analysis APOR anthraquinone extraction of the best technology, and for predictive analysis. Results The optimum extraction conditions of total anthraquinone were as follows: the volume fraction of ethanol was 67.25%, the time was 90 min, the ratio of material to liquid was 28:1; the optimum extraction process of emodin was as follows: the volume fraction of ethanol was 85%, the time was 90 min, 40: 1. Conclusion The Plackett-Burman experimental design combined with CCD to determine the extraction process of APOR total anthraquinone and emodin, the method is simple, accurate, reproducible, and predictive.