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为研究AhHDA1在花生(Arachis hypogaea)干旱胁迫响应中的作用,本文分析了四叶期花生根和叶片在20%(m/V)聚乙二醇6000(PEG6000)模拟干旱胁迫下AhHDA1和干旱响应基因的表达变化,发现AhHDA1、AhAREB1和AhNCED1在叶片中分别上调表达至原来的4.3、2.6和10.3倍,在根中分别下调表达91.1%、87.3%和89.2%。基因表达模式提示AhHDA1可能与脱落酸(ABA)合成和信号传导有关。通过测定干旱胁迫下35S::eGFP、35S::AhHDA1和35S::AhHDA1-RNAi毛状根含水量、ABA含量、抗氧化酶活性、过氧化氢(H_2O_2)含量和丙二醛(MDA)含量,发现35S::AhHDA1-RNAi毛状根具有较高的抗旱性和抗氧化能力,35S::AhHDA1-RNAi在干旱胁迫处理后的含水量为75.3%,脱落酸含量为1.23μg·g~(-1),均显著低于其他两种毛状根,表明抑制AhHDA1表达可提高毛状根细胞的抗旱能力。染色质免疫沉淀(ChIP)分析花生根在干旱胁迫下AhNCED1和AhAREB1启动子区域H3K14ac抗体富集度变化情况,结果表明,AhNCED1和AhAREB1在H3K14位点的乙酰化程度改变分别在转录起始位点(TSS)和近TSS区启动子发生。
In order to study the role of AhHDA1 in the response to drought stress in Arachis hypogaea, we analyzed the effect of AhHDA1 and drought response under drought stress of 20% (m / V) polyethylene glycol 6000 (PEG6000) The results showed that AhHDA1, AhAREB1 and AhNCED1 were up-regulated to 4.3, 2.6 and 10.3 folds in leaves respectively, and down-regulated 91.1%, 87.3% and 89.2% in roots respectively. The pattern of gene expression suggests that AhHDA1 may be involved in abscisic acid (ABA) synthesis and signal transduction. The water content, ABA content, antioxidant enzyme activity, hydrogen peroxide (H 2 O 2) content and malondialdehyde (MDA) content of 35S :: eGFP, 35S :: AhHDA1 and 35S :: AhHDA1-RNAi under drought stress The results showed that the 35S :: AhHDA1-RNAi hairy roots had higher drought resistance and antioxidant capacity. The water content of 35S :: AhHDA1-RNAi after drought stress treatment was 75.3% and the content of ABA was 1.23μg · g ~ (-1) -1) were significantly lower than the other two hairy roots, indicating that inhibition of AhHDA1 expression can improve hairy root cells drought resistance. Chromatin immunoprecipitation (ChIP) analysis of peanut roots under drought stress AhNCED1 and AhAREB1 promoter region H3K14ac antibody enrichment changes, the results showed that AhNCED1 and AhAREB1 at H3K14 acetylation changes in the transcriptional start site (TSS) and near-TSS promoter.